Proximodistal patterning during limb regeneration
Proximodistal patterning during limb regeneration
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DOI：
10.1016/j.ydbio.2004.12.029
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发表时间：
2005-03-15
影响因子：
2.7
通讯作者：
Tanaka, EM
中科院分区：
文献类型：
--
作者：
Echeverri, K;Tanaka, EM
再生是一种能力，已被广泛观察到整个后生动物的生殖发育。在脊椎动物中，有尾目两栖动物因其再生肢体等身体部位的特殊能力而脱颖而出。在这个过程中，只有肢体的缺失部分被精确地保留下来-上臂截肢会导致整个肢体的再生，而手腕截肢会产生一只手。肢体再生是通过形成称为芽基的局部增殖区而发生的。在这里，我们研究如何proximodistal身份是建立在芽基。使用细胞标记和移植实验，我们表明，远端身份已经建立在最早阶段的芽检查。将细胞移植到新的环境中不足以重新指定细胞身份。然而，过度表达的CD 59，细胞表面分子先前牵连在proximodistal身份在肢体再生。导致远端芽基细胞移位到更近端的位置，并导致再生体远端元件的图案缺陷。我们提出了一个肢体再生芽基模型，截肢后4天，芽基已经分为不同的生长区，每个区的细胞已经指定产生上臂，下臂，和手。(C)2005年爱思唯尔公司All rights reserved.
Regeneration is an ability that has been observed extensively throughout metazoan phylogeny. Amongst vertebrates, the urodele amphibians stand out for their exceptional capacity to regenerate body parts such as the limb. During this process, only the missing portion of the limb is precisely replaced-amputation in the Upper arm results in regeneration of the entire limb, while amputation at the wrist produces a hand. Limb regeneration occurs through the formation of a local proliferative zone called the blastema. Here, we examine how proximodistal identity is established in the blastema. Using cell marking and transplantation experiments, we show that distal identities have already been established in the earliest stages of blastemas examined. Transplantation of cells into new environments is not sufficient to respecify cell identity. However, overexpression of the CD59, a cell surface molecule previously implicated in proximodistal identity during limb regeneration. causes distal blastema cells to translocate to a more proximal location and causes defects in the patterning of the distal elements of the regenerate. We suggest a model for the limb regeneration blastema where by 4 days post-amputation the blastema is already divided into distinct growth zones; the cells of each zone are already specified to give rise to upper arm, lower ann, and hand. (C) 2005 Elsevier Inc. All rights reserved.