Selective elimination of messenger RNA prevents an incidence of untimely meiosis
Selective elimination of messenger RNA prevents an incidence of untimely meiosis
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DOI：
10.1038/nature04881
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发表时间：
2006-07-06
期刊：
影响因子：
64.8
通讯作者：
Yamamoto, Masayuki
中科院分区：
文献类型：
--
作者：
Harigaya, Yuriko;Tanaka, Hirotsugu;Yamamoto, Masayuki
关于减数分裂的分子调控仍有许多未知之处。在这里，我们表明，减数分裂特异性转录选择性地删除，如果在分裂酵母的营养生长过程中表达。这些信使RNA包含一个顺式作用区，我们称之为DSR，它通过与YTH家族蛋白Mmi 1结合来实现这种去除。由于减数分裂转录本的不合时宜表达，Mmi 1功能的丧失严重损害细胞生长。微阵列分析显示，至少有十几个这样的减数分裂特异性转录本被DSR-Mmi 1系统消除。mmi 1在营养生长过程中保持多核灶的形式。在减数分裂前期，这些焦点沉淀成一个单一的焦点，这与主减数分裂调节因子Mei 2形成的点相一致。由于Mei 2点的丢失而导致的减数分裂停滞通过Mmi 1活性的降低而释放。我们建议Mei 2关闭DSR-Mmi 1系统通过隔离Mmi 1的点，从而确保稳定表达减数分裂特异性转录本。
Much remains unknown about the molecular regulation of meiosis. Here we show that meiosis-specific transcripts are selectively removed if expressed during vegetative growth in fission yeast. These messenger RNAs contain a cis-acting region-which we call the DSR-that confers this removal via binding to a YTH-family protein Mmi1. Loss of Mmi1 function severely impairs cell growth owing to the untimely expression of meiotic transcripts. Microarray analysis reveals that at least a dozen such meiosis-specific transcripts are eliminated by the DSR-Mmi1 system. Mmi1 remains in the form of multiple nuclear foci during vegetative growth. At meiotic prophase these foci precipitate to a single focus, which coincides with the dot formed by the master meiosis-regulator Mei2. A meiotic arrest due to the loss of the Mei2 dot is released by a reduction in Mmi1 activity. We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts.