Impact of Helicobacter Pylori Infection on Duodenal Microbial Community Structure and Microbial Metabolic Pathways
Impact of Helicobacter Pylori Infection on Duodenal Microbial Community Structure and Microbial Metabolic Pathways
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DOI：
10.21203/rs.3.rs-166718/v1
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发表时间：
2020-12
影响因子：
4.2
通讯作者：
Tadashi Maeda;Hiroaki Zai;Y. Fukui;Y. Kato;Eri Kumade;Toshiyasu Watanabe;N. Furusyo;H. Nakajima;K. Arai;Y. Ishii;K. Tateda;Y. Urita
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文献类型：
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作者：
Tadashi Maeda;Hiroaki Zai;Y. Fukui;Y. Kato;Eri Kumade;Toshiyasu Watanabe;N. Furusyo;H. Nakajima;K. Arai;Y. Ishii;K. Tateda;Y. Urita
研究背景最近的研究表明，幽门螺杆菌感染可能与某些疾病的发生有关。然而，在这些疾病的病因中发挥作用的幽门螺杆菌相关因素尚未完全阐明。本研究旨在阐明幽门螺杆菌感染对十二指肠共生菌群结构及其生物学功能的影响。结果13例Hp阳性，34例Hp阴性。共观察到细菌操作分类单位1404个，隶属于23门253属。在幽门螺杆菌阳性组中，我们观察到与幽门螺杆菌阴性组相比，蛋白杆菌的丰度更高，放线杆菌和TM7的丰度更低。幽门螺杆菌阳性组与阴性组10个属的丰度有显著差异。幽门螺杆菌阳性组的微生物区系特征受到12个主要属于伽玛变形杆菌的分类群的显著影响。利用京都百科全书基因和基因组正交学数据库整理的微生物功能注释显示，12条微生物代谢途径(酮体的合成和降解、色氨酸代谢、N-葡聚糖生物合成、乙醚脂肪代谢、脂阿拉伯甘露聚糖(LAM)生物合成、亚油酸代谢、α-亚麻酸代谢、生物素代谢、类胡萝卜素生物合成、苯丙素生物合成、铁载体非核糖体多肽的生物合成、萜类和类固醇的生物合成)显著受幽门螺杆菌感染的影响。幽门螺杆菌感染扰乱了十二指肠内正常的细菌群落，并改变了共生微生物区系的生物功能，主要是通过上调特定的代谢途径。这种改变可能与怀疑与幽门螺杆菌感染有关的疾病的发病机制有关。
BackgroundRecent reports suggest that Helicobacter pylori infection may be related to the onset of certain diseases. However, the H. pylori-related factors that play a role in the etiology of these diseases have not been fully elucidated. This study aimed to elucidate the impact of H. pylori infection on the structure of commensal duodenal microbiota and their biofunctions.MethodsForty-seven (20 male, 27 female) subjects who underwent gastric cancer screening were enrolled. Duodenal fluid samples were aspirated from the descending duodenum and analyzed by 16S rRNA gene sequencing.ResultsThirteen subjects were positive for H. pylori, while thirty-four were negative. We observed 1404 bacterial operational taxonomic units from 23 phyla and 253 genera. In the H. pylori-positive group, we observed higher abundances of Proteobacteria and lower abundances of Actinobacteria and TM7 than that in the H. pylori-negative group. The abundances of 10 genera differed significantly between the H. pylori-positive and -negative groups. Microbiota features in the H. pylori-positive group was significantly influenced by 12 taxa primarily belonging to Gammaproteobacteria. Microbial functional annotation collated using the Kyoto Encyclopedia of Genes and Genomes Orthology database showed that 12 microbial metabolic pathways (Synthesis and degradation of ketone bodies, Tryptophan metabolism, N-glycan biosynthesis, Ether lipid metabolism, Lipoarabinomannan (LAM) biosynthesis, Linoleic acid metabolism, alpha-Linolenic acid metabolism, Biotin metabolism, Carotenoid biosynthesis, Phenylpropanoid biosynthesis, Biosynthesis of siderophore group nonribosomal peptides, Biosynthesis of terpenoids and steroids) were significantly affected by H. pylori infection.ConclusionsH. pylori infection disrupted the normal bacterial communities in the duodenum and changed the biofunctions of the commensal microbiota, primarily by upregulating specific metabolic pathways. This alteration may be related to the onset mechanisms of the diseases suspected of being related to H. pylori infection.