Interferon‐Induced NK Augmentation in Humans
Interferon‐Induced NK Augmentation in Humans
复制标题
干扰素诱导的人类 NK 增强
DOI：
10.1111/j.1365-3083.1981.tb00566.x
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发表时间：
1981
影响因子：
3.7
通讯作者：
M. Jondal
中科院分区：
文献类型：
--
作者：
M. Ullberg;J. Merrill;M. Jondal
通过将琼脂糖中的单细胞细胞毒性试验与通过51 Cr释放估计的最大自然杀伤（NK）细胞潜力（Vmax）相结合，分析了干扰素增强人NK细胞的机制。研究了短期干扰素治疗后靶结合细胞（TBC）的数量、活性TBC的比例以及NK细胞的回收。结果表明干扰素对人NK细胞的双重作用：效应细胞募集和增加效应细胞再循环。当针对标准靶K-562和源自B-型淋巴瘤的Daudi和BJAB细胞检测NK细胞时，这两个变量均增加。然而，当将源自急性淋巴细胞白血病的T细胞系（Molt-4和1301）用作靶细胞时，在未治疗的TBC中发现了较大比例的活性NK细胞，而干扰素治疗仅导致效应细胞再循环增加，而不是效应细胞募集。干扰素治疗后，任何检测的细胞系均未检测到TBCs增加。T细胞系和非T细胞系在干扰素诱导的效应细胞募集方面的差异与人类NK系统的已知特征有关。
By combining a single‐cell cytotoxicity assay in agarose with estimations of the maximal natural killer (NK) cell potential (Vmax) by 51Cr release, the mechanisms behind interferon augmentation of human NK cells were analysed. The number of target‐binding cells (TBCs) the fraction of active TBCs, and NK cell recycling were studied after short‐term interferon treatment. The results demonstrate a dual effect of interferon on human NK cells: effector cell recruitment and increased effector cell recycling. Both of these variables were increased when NK cells were tested against the standard target K‐562 and against Daudi and BJAB cells, derived from B‐type lymphomas. However, when T cell lines derived from acute lymphocytic leukaemia (Molt‐4 and 1301) were used as targets, a larger fraction of active NK cells were found among untreated TBCs, whereas inteferon treatment only resulted in increased effector cell recycling and not in effector cell recruitment. No increase in TBCs after interferon treatment could be detected with any cell line tested. The difference seen between T and non‐T cell lines with regard 10 interferon‐induced effector cell recruitment is discussed in relation to known characteristics of the human NK system.