Clinical Sequencing Uncovers Origins and Evolution of Lassa Virus.
Clinical Sequencing Uncovers Origins and Evolution of Lassa Virus.
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DOI：
10.1016/j.cell.2015.07.020
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发表时间：
2015-08-13
期刊：
影响因子：
64.5
通讯作者：
Sabeti PC
中科院分区：
文献类型：
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作者：
Andersen KG;Shapiro BJ;Matranga CB;Sealfon R;Lin AE;Moses LM;Folarin OA;Goba A;Odia I;Ehiane PE;Momoh M;England EM;Winnicki S;Branco LM;Gire SK;Phelan E;Tariyal R;Tewhey R;Omoniwa O;Fullah M;Fonnie R;Fonnie M;Kanneh L;Jalloh S;Gbakie M;Saffa S;Karbo K;Gladden AD;Qu J;Stremlau M;Nekoui M;Finucane HK;Tabrizi S;Vitti JJ;Birren B;Fitzgerald M;McCowan C;Ireland A;Berlin AM;Bochicchio J;Tazon-Vega B;Lennon NJ;Ryan EM;Bjornson Z;Milner DA Jr;Lukens AK;Broodie N;Rowland M;Heinrich M;Akdag M;Schieffelin JS;Levy D;Akpan H;Bausch DG;Rubins K;McCormick JB;Lander ES;Günther S;Hensley L;Okogbenin S;Viral Hemorrhagic Fever Consortium;Schaffner SF;Okokhere PO;Khan SH;Grant DS;Akpede GO;Asogun DA;Gnirke A;Levin JZ;Happi CT;Garry RF;Sabeti PC
2013-2015年西非埃博拉病毒病（EVD）疫情提醒我们，人们对生物安全4级病毒知之甚少。与埃博拉病毒一样，拉沙病毒（LASV）可引起出血热，病死率很高。我们从临床和啮齿动物储存样本中生成了近 200 个 LASV 序列的基因组目录。我们发现，2013-2015 年埃博拉病毒病疫情是由人际传播推动的，而 LASV 感染主要是由病毒宿主传染给人类造成的。我们阐明了 LASV 在西非的传播，并表明这种迁移伴随着 LASV 基因组丰度、死亡率、密码子适应和翻译效率的变化。通过研究宿主内进化，我们发现突变在病毒表面蛋白的表位中积累，这表明免疫逃逸的选择。该目录将作为疫苗和诊断方法开发的基础。
The 2013-2015 West African epidemic of Ebola virus disease (EVD) reminds us how little is known about biosafety level-4 viruses. Like Ebola virus, Lassa virus (LASV) can cause hemorrhagic fever with high case fatality rates. We generated a genomic catalog of almost 200 LASV sequences from clinical and rodent reservoir samples. We show that whereas the 2013-2015 EVD epidemic is fueled by human-to-human transmissions, LASV infections mainly result from reservoir-to-human infections. We elucidated the spread of LASV across West Africa and show that this migration was accompanied by changes in LASV genome abundance, fatality rates, codon adaptation, and translational efficiency. By investigating intrahost evolution, we found that mutations accumulate in epitopes of viral surface proteins, suggesting selection for immune escape. This catalog will serve as a foundation for the development of vaccines and diagnostics.