Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2
Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2
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DOI：
10.1039/c5bm00231a
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发表时间：
2015-01-01
影响因子：
6.6
通讯作者：
Asanuma, Hiroyuki
中科院分区：
文献类型：
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作者：
Kamiya, Yukiko;Iishiba, Kazuki;Asanuma, Hiroyuki
我们报道了在每个末端用对氰基二苯乙烯修饰的光交联型siRNA链的发展。该siRNA具有抗核酸酶活性，并保持了RNAi活性。我们进一步研究了共价交联siRNA的激活机制。有趣的是，已知能从前体siRNA中产生带有突出3‘端的siRNA的DICER根本没有切割交联型siRNA。我们的结果表明，交联型siRNAs的激活需要ArgAerte2的切割。
We report the development of photo-crosslinked siRNA strands modified at each terminus with p-cyanostilbene. The siRNA was nuclease resistant and retained RNAi activity. We further studied the activation mechanism of the covalently-crosslinked siRNA. Interestingly Dicer, which is known to generate siRNA with overhanging 3' ends from the precursor siRNA, did not cleave the crosslinked siRNA at all. Our results suggest that the activation of the crosslinked siRNAs required cleavage by Argonaute2.