Identification and characterization of a novel paramyxovirus, porcine parainfluenza virus 1, from deceased pigs
Identification and characterization of a novel paramyxovirus, porcine parainfluenza virus 1, from deceased pigs
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DOI：
10.1099/vir.0.052985-0
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发表时间：
2013-10-01
影响因子：
3.8
通讯作者：
Yuen, Kwok-Yung
中科院分区：
文献类型：
--
作者：
Lau, Susanna K. P.;Woo, Patrick C. Y.;Yuen, Kwok-Yung
我们描述了一种新的副粘病毒，猪副流感病毒1（PPIV-1），从猪的发现和表征。通过逆转录-PCR在386头死亡猪的386份鼻咽拭子样本中的12份（3.1%）和303份直肠拭子样本中的2份（0.7%）中检测到病毒，病毒载量高达10（6）拷贝ml（-1）。全基因组测序和系统发育分析表明，PPIV-1代表呼吸道病毒属内的一种新副粘病毒，与人副流感病毒1型（HPIV-1）和仙台病毒（SeV）关系最密切。与HPIV-1相比，PPIV-1在磷蛋白基因中具有mRNA编辑功能。此外，PPIV-1在呼吸道病毒中是独特的，在编辑位点A（6）运行后有两个G残基，而不是三到五个G残基。然而，PPIV-1、HPIV-1和SeV具有共同的基因组特征，并且可能属于呼吸道病毒属内的单独组。主要呼吸道样本中存在PPIV-1表明可能与呼吸道疾病相关，与HPIV-1和SeV相似。
We describe the discovery and characterization of a novel paramyxovirus, porcine parainfluenza virus 1 (PPIV-1), from swine. The virus was detected in 12 (3.1%) of 386 nasopharyngeal and two (0.7%) of 303 rectal swab samples from 386 deceased pigs by reverse transcription-PCR, with viral loads of up to 10(6) copies ml(-1). Complete genome sequencing and phylogenetic analysis showed that PPIV-1 represented a novel paramyxovirus within the genus Respirovirus, being most closely related to human parainfluenza virus 1 (HPIV-1) and Sendai virus (SeV). In contrast to HPIV-1, PPIV-1 possessed a mRNA editing function in the phosphoprotein gene. Moreover, PPIV-1 was unique among respiroviruses in having two G residues instead of three to five G residues following the A(6) run at the editing site. Nevertheless, PPIV-1, HPIV-1 and SeV share common genomic features and may belong to a separate group within the genus Respirovirus. The presence of PPIV-1 in mainly respiratory samples suggests a possible association with respiratory disease, similar to HPIV-1 and SeV.