Mystery of intron gain
Mystery of intron gain
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DOI：
10.1101/gr.1029803
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发表时间：
2003-10-01
期刊：
影响因子：
7
通讯作者：
Gilbert, W
中科院分区：
文献类型：
--
作者：
Fedorov, A;Roy, S;Gilbert, W
近15年来，人们普遍认为许多内含子是最近由多细胞生物的基因获得的。然而，对于单个动物内含子的获得机制还有待描述。在这里，我们报告了一个大规模的计算分析的人类，果蝇，秀丽隐杆线虫，拟南芥基因组。我们将147，796个人类内含子序列分成相似长度的批次，并将它们彼此对齐。不同类型的内含子之间的同源性被发现，但没有一个简单的内含子转座的证据。此外，106，902植物，39，624果蝇和6021线虫内含子进行了检查。在非同源基因中没有发现同源内含子。因此，在过去的5000万年里，我们没有发现人类内含子转座的例子，在果蝇和线虫的300万年里，或者在拟南芥的500万年里。要么新的内含子不是通过其他内含子的转座而产生的，要么内含子的转座一定是在进化的早期发生的，以至于所有同源性的痕迹都消失了。
For nearly 15 years, it has been widely believed that many introns were recently acquired by the genes of multicellular organisms. However, the mechanism Of acquisition has yet to be described for a single animal intron. Here, we report a large-scale computational analysis of the human, Drosophila melanogaster, Caenorhabditis elegans, and Arabidopsis thaliana genomes. We divided 147,796 human intron sequences into batches of similar lengths and aligned them with each other. Different types of homologies between introns were found, but none showed evidence of simple intron transposition. Also, 106,902 plant, 39,624 Drosophila, and 6021 C elegans introns were examined. No single case of homologous introns in nonhomologous genes was detected. Thus, we found no example of transposition of introns in the last 50 million years in humans, in 3 million years in Drosophila and C elegans, or in 5 million years in Arabidopsis. Either new introns do not arise via transposition of other introns or intron transposition must have occurred so early in evolution that all traces of homology have been lost.