Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan.
Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan.
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DOI：
10.1038/srep20213
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发表时间：
2016-02-11
影响因子：
4.6
通讯作者：
Hayasaka D
中科院分区：
文献类型：
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作者：
Shimada S;Aoki K;Nabeshima T;Fuxun Y;Kurosaki Y;Shiogama K;Onouchi T;Sakaguchi M;Fuchigami T;Ono H;Nishi K;Posadas-Herrera G;Uchida L;Takamatsu Y;Yasuda J;Tsutsumi Y;Fujita H;Morita K;Hayasaka D
粘蜱传播几种重要的病毒病原体。我们从日本的黄血蜱和形血蜱中分离到了一种新的病毒（Tofla病毒：TFLV）。全基因组测序结果表明，该病毒属于布尼亚病毒科奈罗病毒属。系统发育分析和中和试验表明，TFLV与哈扎拉病毒密切相关，并被归类为克里米亚-刚果出血热组。TFLV引起IFNAR KO小鼠的致死性感染。感染tflv的小鼠表现出胃肠道紊乱，正电子发射断层扫描-计算机断层扫描图像显示肠道中明显摄取18f -氟脱氧葡萄糖。TFLV能够感染非洲绿猴来源的Vero E6细胞和人来源的SK-N-SH、T98-G和HEK-293细胞并在培养细胞中繁殖。虽然人类和动物的TFLV感染目前尚不清楚，但我们的发现可能为了解潜在的传染性和制定针对这种新的蜱传奈罗病毒的先发制人的对策提供线索。
Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of 18F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.