MXA GENE-EXPRESSION AFTER LIVE VIRUS VACCINATION - A SENSITIVE MARKER FOR ENDOGENOUS TYPE-I INTERFERON
MXA GENE-EXPRESSION AFTER LIVE VIRUS VACCINATION - A SENSITIVE MARKER FOR ENDOGENOUS TYPE-I INTERFERON
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DOI：
10.1093/infdis/169.4.807
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发表时间：
1994-04-01
影响因子：
6.4
通讯作者：
HALLER, O
中科院分区：
文献类型：
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作者：
ROERS, A;HOCHKEPPEL, HK;HALLER, O
众所周知，MxA基因的表达受到I型干扰素(IFN)的严格和专一性的调控。对11名健康志愿者接种17-D株黄热病病毒后外周血单个核细胞中MxA基因表达的动态变化进行了分析。在缺乏易于检测到的血清干扰素活性的情况下，可以可靠地诱导MxA RNA和MxA蛋白。因此，在接种疫苗后第5天，稳态MX4 RNA水平比预防水平高8-30倍。MxA蛋白的平均增幅约为50倍。相比之下，在3名因之前接种疫苗而免疫的相似疫苗接种的对照组中，没有检测到MxA RNA或MxA蛋白的诱导。已知对I型和II型IFN有反应的干扰素标志物2‘-5’-寡腺苷(2-5A)合成酶显示出类似的反应，但在非免疫者和免疫者之间不能很好地区分。β(2)-微球蛋白和新喋呤的反应很差，保持在正常范围内的低水平。这些结果表明，MxA基因的表达是检测病毒感染过程中微量具有生物活性的I型干扰素的良好标志。
MxA gene expression is known to be regulated tightly and exclusively by type I interferons (IFNs). The kinetics of MxA gene expression was analyzed in peripheral blood mononuclear cells from 11 healthy volunteers vaccinated with the 17-D strain of yellow fever virus. A reliable induction of MxA RNA and MxA protein was found in the absence of easily detectable serum IFN activity. Thus, steady-state Mx4 RNA levels were elevated 8- to 30-fold above prevaccination levels on day 5 after vaccination. The average increase of MxA protein was similar to 50-fold. In contrast, no induction of MxA RNA or MxA protein was detectable in 3 similarly vaccinated controls who were immune because of previous vaccinations. The IFN marker 2'-5'-oligoadenylate (2-5A) synthetase known to react to both type I and type II IFNs showed a similar response but did not differentiate equally well between nonimmune and immune vaccinees. beta(2)-micraglobulin and neopterin reacted poorly, remaining at low levels within the normal range. These results demonstrate that MxA gene expression is a good marker for detecting minute quantities of biologically active type I IFN during viral infections.