STEADY-STATE CONCENTRATIONS OF MESSENGER-RIBONUCLEIC-ACID ENCODING CYTOCHROME-P450 SIDE-CHAIN CLEAVAGE AND 3-BETA-HYDROXYSTEROID DEHYDROGENASE DELTA-5,DELTA-4 ISOMERASE IN OVINE CORPORA-LUTEA DURING THE ESTROUS-CYCLE
STEADY-STATE CONCENTRATIONS OF MESSENGER-RIBONUCLEIC-ACID ENCODING CYTOCHROME-P450 SIDE-CHAIN CLEAVAGE AND 3-BETA-HYDROXYSTEROID DEHYDROGENASE DELTA-5,DELTA-4 ISOMERASE IN OVINE CORPORA-LUTEA DURING THE ESTROUS-CYCLE
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DOI：
10.1095/biolreprod51.3.380
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发表时间：
1994-09-01
影响因子：
3.6
通讯作者：
NISWENDER, GD
中科院分区：
文献类型：
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作者：
JUENGEL, JL;GUY, MK;NISWENDER, GD
这些实验的目的是确定发情周期第3、6、9、12和15天卵泡黄体化期间和绵羊黄体组织中细胞色素P450侧链裂解（P450（SCC））和3 β-羟基类固醇脱氢酶/δ（5）、δ（4）异构酶（3 β-HSD）的mRNA表达模式。在发情开始后4小时或24小时收集的卵泡中，P450（SCC）的平均mRNA浓度没有差异，但到48小时（卵巢体）时增加了3倍（p < 0.05）。使用所用的方法，SP-HSD的mRM 1直到排卵和黄体形成后（发情开始后48小时）才被检测到。在黄体组织中，P450（SCC）的平均mRNA浓度从第3天到第3天增加（P < 0.05），并且到第15天降低（P < 0.05）。第3天小黄体细胞中P450（SCC）mRNA的平均浓度高于第15天（P < 0.05），第6天和第12天的值居中。在大的黄体细胞中，P450（SCC）mRNA的平均浓度在第6 - 12天增加（P < 0.05），然后在第15天下降（P < 0.05）。在检查的任何一天，黄体组织中3 β-HSD的平均mRNA浓度没有差异（P = 0.33）。在小的黄体细胞中，3 β-HSD的mRNA的平均浓度在第6天和第15天之间降低（p < 0.05），而在大的黄体细胞中，平均浓度在第12天和第15天之间降低（p < 0.05）。总之，P450（SCC）和3 β-HSD mRNA的上调发生在排卵后。在CL中，P450（SCC）的mRNA浓度从第3天至第9天增加，然后在第15天再次下降。相反，3 β-HSD的mRNA浓度在发情周期中没有变化。在本实验中，在发情周期的大部分时间里，大黄体细胞比小黄体细胞具有更高浓度的P450（SCC）和3 β-HSD mRNA。
The objective of these experiments was to determine the pattern of mRNA expression for cytochrome P450 side-chain cleavage (P450(scc)) and 3 beta-hydroxysteroid dehydrogenase/Delta(5),Delta(4) isomerase (3 beta-HSD) during luteinization of the follicle and in ovine luteal tissue on Days 3, 6, 9, 12, and 15 of the estrous cycle. Mean concentration of mRNA for P450(scc) was not different in follicles collected 4 or 24 h after the onset of estrus but increased (p < 0.05) 3-fold by 48 h (corpus hemorrhagicum). With the methods used, mRM1 for SP-HSD was not detected until after ovulation and formation of the corpus hemorrhagicum (48 h after onset of estrus). In luteal tissue, mean concentration of mRNA for P450(scc) increased from Days 3 to 3 (P < 0.05) and had decreased (p < 0.05) by Day 15. Mean concentration of mRNA for P450(scc) was higher (P < 0.05) in small luteal cells on Day 3 than on Day 15, with values on Days 6 and 12 being intermediate. In large luteal cells, mean concentrations of P450(scc) mRNA increased (p < 0.05) between Days 6 and 12 and then decreased (P < 0.05) on Day 15. Mean concentration of mRNA for 3 beta-HSD was not different (P = 0.33) in luteal tissue on any day examined. In small luteal cells, mean concentrations of mRNA for 3 beta-HSD decreased between Days 6 and 15 (p < 0.05) while in large luteal cells, mean concentrations decreased (p < 0.05) between Days 12 and 15. In summary, up-regulation of mRNA for P450(scc) and 3 beta-HSD occurred after ovulation. In the CL, concentrations of mRNA for P450(scc) increased from Days 3 to 9 and then decreased again by Day 15. In contrast, concentrations of mRNA for 3 beta-HSD did not change during the estrous cycle. Large luteal cells had higher concentrations of mRNA for P450(scc) and 3 beta-HSD than small luteal cells on most days of the estrous cycle examined in this experiment.