A 2-COMPONENT SYSTEM THAT REGULATES AN OSMOSENSING MAP KINASE CASCADE IN YEAST
A 2-COMPONENT SYSTEM THAT REGULATES AN OSMOSENSING MAP KINASE CASCADE IN YEAST
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DOI：
10.1038/369242a0
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发表时间：
1994-05-19
期刊：
影响因子：
64.8
通讯作者：
SAITO, H
中科院分区：
文献类型：
--
作者：
MAEDA, T;WURGLERMURPHY, SM;SAITO, H
在原核双组分信号转导系统中，传感器分子对环境刺激的识别导致其组氨酸激酶结构域的激活和该结构域内组氨酸残基的磷酸化（1-3）。然后，该磷酸基团转移到同源反应调节分子的受体结构域中的天冬氨酸残基，导致其输出功能的激活。尽管最近鉴定出一些真核生物蛋白质显示出与原核生物传感器或反应调节器的序列相似性，但尚不清楚它们是否构成“双组分”系统的一部分（4-7）。在这里，我们描述了酿酒酵母中的一个双组分系统，该系统调节一个MAP激酶级联反应（8，9）。
IN the prokaryotic two-component signal transduction systems, recognition of an environmental stimulus by a sensor molecule results in the activation of its histidine kinase domain and phosphorylation of a histidine residue within that domain(1-3). This phosphate group is then transferred to an aspartate residue in the receiver domain of a cognate response regulator molecule, resulting in the activation of its output function. Although a few eukaryotic proteins were identified recently that show sequence similarity to the prokaryotic sensors or response regulators, it has not been clear whether they constituted a part of a 'two-component' system(4-7). Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade(8,9).