Controlling mammalian cell spreading and cytoskeletal arrangement with conveniently fabricated continuous wavy features on poly(dimethylsiloxane)
Controlling mammalian cell spreading and cytoskeletal arrangement with conveniently fabricated continuous wavy features on poly(dimethylsiloxane)
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DOI：
10.1021/la011668
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发表时间：
2002-04-16
期刊：
影响因子：
3.9
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Jiang, XY;Takayama, S;Whitesides, GM
本文报道了一种简单而通用的技术，用于在聚（二甲基硅氧烷）（PDMS）的表面上产生结构，周期在0.1和10 μ m之间的近似正弦波，并使用这些结构来研究细胞接触指导。这些特征是通过机械拉伸PDMS板，在氧等离子体中氧化它们，并使它们松弛而产生的。这些表面特征类似于传统上用于研究细胞接触引导的光刻制造的凹槽，尽管它们的边缘是圆形的而不是角形的。牛毛细血管内皮细胞在这些特征上排列和伸长。对齐的细胞的形态和细胞骨架结构与先前在具有其他类型的形貌的表面上的接触引导的研究中描述的细胞的形态和细胞骨架结构相似。这些观察结果和比较表明，定义凹槽的特征中的锐边在引起接触引导方面并不重要。该技术提供了一种用于制造微特征的方法，用于研究细胞与其环境之间的相互作用，而不需要洁净室或使用清洁工具。
This paper reports a simple and versatile technique for generating structures on the surfaces of poly(dimethylsiloxane) (PDMS), approximately sinusoidal waves with periods between 0.1 and 10 mum, and the use of these structures to study cell contact guidance.' The features are generated by stretching PDMS slabs mechanically, oxidizing them in an oxygen plasma, and allowing them to relax. These surface features are similar to photolithographically fabricated grooves that have traditionally been used to investigate cell contact guidance, although their edges are rounded rather than angular. Bovine capillary endothelial cells align and elongate on these features. The morphology and cytoskeletal structure of the aligned cells are similar to those of cells described in previous studies of contact guidance on surfaces with other types of topography. These observations and comparisons indicate that sharp edges in the features defining the grooves are not essential in eliciting contact guidance. This technique provides a method for fabricating microfeatures for the studies of the interactions between cells and their environment that does not require a cleanroom or access to photolithographic tools.