Gene expression profiling of human atrial myocardium with atrial fibrillation by DNA microarray analysis
Gene expression profiling of human atrial myocardium with atrial fibrillation by DNA microarray analysis
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DOI：
10.1016/j.ijcard.2004.05.026
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发表时间：
2005-07-10
影响因子：
3.5
通讯作者：
Shimada, K
中科院分区：
文献类型：
--
作者：
Ohki, R;Yamamoto, K;Shimada, K
背景：心房颤动（AF）是临床上最常见的心律失常。然而，AF的分子机制尚未进行全面的调查。本研究的目的是澄清基因转录谱调制的AF patients using DNA microarray technology.Methods：我们获得了17个新鲜的心脏标本，右心耳，分离自10例正常窦性心律和7例慢性AF患者接受心脏手术。每个样本使用了研究12，000个人类基因的Affyssin GeneChip（人类基因组U95 A）。结果：房颤组左房内径大于窦性心律组，房颤组左房内径大于窦性心律组。与窦性心律组相比，我们可以鉴定出33个AF特异性基因被显著激活（> 1.5倍），包括离子通道，抗氧化剂，炎症，三个细胞生长/细胞周期，三个转录如核因子-白细胞介素6-β，几个细胞信号传导和几个蛋白质基因，以及七个表达序列标签（EST）。相反，我们发现了63个窦性心律特异性基因，包括几个细胞信号/通信，如肌浆网Ca 2 +-ATP酶2，几个细胞呼吸和能量产生和两个抗增殖或负调节细胞生长基因，和22 ESTs.Conclusions：本研究表明，约100个基因在房颤患者的心房调控。这些发现表明，这些基因可能在房颤的发生或持续以及心房重构的病理生理学中起关键作用。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
Background: Atria] fibrillation (AF) is the most frequently encountered arrhythmia in the clinical setting. However, a comprehensive investigation of the molecular mechanism of AF has not been performed. The aim of this study was to clarify transcriptional profiling of genes modulated in the atrium of AF patients using DNA microarray technology.Methods: We obtained 17 fresh cardiac specimens, right atrial appendages, isolated from 10 patients with normal sinus rhythm and seven chronic AF patients who underwent cardiac surgery. Affymetrix GeneChip (Human Genome U95A) investigating 12,000 human genes was used for each specimen. Quantitative analysis of selected genes was performed by the real-time PCR method.Results: The left atrial diameter in the AF group was greater than that in the sinus rhythm group. We could identify 33 AF-specific genes that were significantly activated (> 1.5-fold), compared with the sinus rhythm group, including an ion channel, an antioxidant, an inflammation, three cell growth/cell cycle, three transcription such as nuclear factor-interleukin 6-beta, several cell signaling and several protein genes, and seven expressed sequence tags (ESTs). In contrast, we found 63 sinus rhythm-specific genes, including several cell signaling/communication such as sarcoplasmic reticulum Ca2+-ATPase 2, several cellular respiration and energy production and two antiproliferative or negative regulator of cell growth genes, and 22 ESTs.Conclusions: The present study demonstrated that about one hundred genes were modulated in the atria of AF patients. These findings suggest that these genes may play critical roles in the initiation or perpetuation of AF and the pathophysiology of atrial remodeling. (c) 2004 Elsevier Ireland Ltd. All rights reserved.