Biomedical application of surface plasmon resonance biosensors (review)
Biomedical application of surface plasmon resonance biosensors (review)
复制标题
表面等离子体共振生物传感器的生物医学应用（综述）
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ingrida Lapënaitë
中科院分区：
文献类型：
--
作者：
A. Ramanavièius;F. Herberg;S. Hutschenreiter;B. Zimmermann;Ingrida Lapënaitë
在过去的十年中，表面等离子体共振（SPR）成为生物化学、生物分析化学和生物医学中检测和研究基于亲和力的相互作用的主要方法之一。过去几年，SPR 在生物医学应用中的巨大发展是本综述重点关注 SPR 在生物医学领域的应用的原因。生物医学应用利用 SPR 的精湛灵敏度来确定 DNA 杂交、诊断病毒引起的疾病、酶-底物相互作用、多克隆抗体表征、表位作图、蛋白质构象研究和无标记免疫测定。本综述还涵盖了表面等离子共振生物传感器发展的一些方面。介绍了 SPR 传感器作用的一般原理，并描述了主要的 SPR 格式。对 SPR 生物传感器用于检测从小有机物到大蛋白质复合物等各种分析物的应用进行了综述。简要描述了 SPR 生物传感器设计中使用的固定方法。讨论了 SPR 在分析作为真核细胞信号生成和转导主要成分的蛋白激酶方面的应用。预测并讨论了SPR应用的新方法和混合SPR传感器的新设计。结论是，提高检测限、多通道性能、开发先进的识别元件是开发新型SPR传感器的主要任务。
Over the last decade, surface plasmon resonance (SPR) becomes one of the major methods for the detection and investigation of affinity-based interactions in biochemistry, bioanalytical chemistry and biomedicine. The tremendous development of SPR use in biomedical applications during the last years is the reason why attention in this review is focused on the application of SPR for biomedical purposes. Biomedical applications take advantage of the exquisite sensitivity of SPR for the determination of DNA hybridization, diagnosis of virus-induced diseases, enzyme–substrate interactions, polyclonal antibody characterization, epitope mapping, protein conformation studies and label-free immunoassays. This review covers also some aspects of the evolution of surface plasmon resonance biosensors. The general principles of SPR sensor action are presented and major SPR formats are described. SPR biosensor employment for detection of various analytes ranging from small organics up to large protein complexes is reviewed. Immobilization methods used in the design of SPR biosensors are briefly described. Application of SPR for analysis of protein kinases as a major component in signal generation and transduction in eukaryotic cell is discussed. New ways of SPR application and new designs of hybrid SPR sensors are predicted and discussed. It is concluded that improvement of detection limits, multichannel performance, development of advanced recognition elements are the major tasks in developing new SPR sensors.
影响因子：
12.6
作者：
Hsieh, HV;Pfeiffer, ZA;Pitner, JB
通讯作者：
Pitner, JB