Low-cost, high-speed identification of counterfeit antimalarial drugs on paper
Low-cost, high-speed identification of counterfeit antimalarial drugs on paper
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DOI：
10.1016/j.talanta.2014.05.050
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发表时间：
2014-12-01
期刊：
影响因子：
6.1
通讯作者：
Remcho, Vincent T.
中科院分区：
文献类型：
--
作者：
Koesdjojo, Myra T.;Wu, Yuanyuan;Remcho, Vincent T.
随着东南亚和撒哈拉以南非洲出现假冒青蒿琥酯抗疟疾药物，我们提出生产一种快速，廉价和简单的基于比色的检测试剂盒，用于检测假冒青蒿琥酯，以保护生命并防止多药耐药性疟疾的发展。该试剂盒基于纸微流体工作，与传统微流体相比具有几个优点，并且具有产生廉价，易于使用，快速和一次性诊断设备的巨大潜力。在这里，我们已经开发了一种比色测定法，该方法对青蒿琥酯具有特异性，并在加入样品后变黄。该测试可以在几分钟内完成，并允许通过比较试纸上显色的黄色与试剂盒附带的彩色编码关键图表来对青蒿琥酯片剂进行半定量分析。更准确和精确的分析是通过使用iPhone相机上的颜色分析仪来完成的，该分析仪测量纸芯片上显影颜色的颜色强度。拍摄芯片的数字图像，并通过测量在纸圈上显影的颜色的平均灰度强度来分析。生成青蒿琥酯浓度对平均灰度强度的图。结果表明，在纸上测试时产生的黄色强度是一致的，并且与样品中存在的青蒿琥酯的量成比例。青蒿琥酯的浓度范围为0.0至20 mg/mL，线性校准图得到检测限为0.98 mg/mL。(C)© 2014 Elsevier B. V.保留所有权利。
With the emergence of artesunate antimalarial counterfeiting in Southeast Asia and sub-Saharan Africa, we present the production of a rapid, inexpensive and simple colorimetric-based testing kit for the detection of counterfeit artesunate in order to preserve life and prevent the development of multi-drug resistant malaria. The kit works based on paper microfluidics which offer several advantages over conventional microfluidics, and has great potential to generate inexpensive, easy-to-use, rapid and disposable diagnostic devices. Here, we have developed a colorimetric assay that is specific to artesunate and turns yellow upon addition of the sample. The test can be done within minutes, and allows for a semi-quantitative analysis of the artesunate tablets by comparing the developed yellow color on the paper test to a color-coded key chart that comes with the kit. A more accurate and precise analysis is done by utilizing a color analyzer on an iPhone camera that measures the color intensity of the developed color on the paper chip. A digital image of the chip was taken and analyzed by measuring the average gray intensity of the color developed on the paper circle. A plot of the artesunate concentration versus the average gray scale intensity was generated. Results show that the intensity of the yellow color developed on the paper test was consistent and proportional to the amount of artesunate present in the sample. With artesunate concentrations ranging from 0.0 to 20 mg/mL, a linear calibration plot was obtained with a detection limit of 0.98 mg/mL. (C) 2014 Elsevier B.V. All rights reserved.