An ultracentrifugation analysis of two hundred fish genomes
An ultracentrifugation analysis of two hundred fish genomes
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DOI：
10.1016/s0378-1119(02)00733-3
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发表时间：
2002-08-07
期刊：
影响因子：
3.5
通讯作者：
Bernardi, G
中科院分区：
文献类型：
--
作者：
Bucciarelli, G;Bernardi, G;Bernardi, G
本研究的目的是提供一个全面的观点的组成特征的鱼类基因组。因此，我们扩大了鱼类的数量，我们已经探索到目前为止，在他们的DNA分析超浓缩氯化铯密度梯度从122到201。这项研究包括来自板鳃类9个目中的3个目（鲨鱼和鳐）、两个dipnoan肺鱼目和两个chondrosteans目（鲟鱼和bichirs）的代表。我们还研究了38个硬骨鱼目中的19个，它们代表了硬骨鱼亚门中除4个（次要）总目之外的所有总目，该亚门包括约23，600种（占所有现存鱼类的96%）。这留下了两个子类，Holocephali（嵌合体）和Coelacanthimorpha（gombessas）。尽管分析的物种和目的数量显著增加，但所有平均性质（模态浮力密度rho（0）、平均浮力密度、CsCl剖面不对称性A和组成异质性H）及其所有范围与先前的研究相比均未发生变化[J. Mol. Biol.评价31（1990）265]。这表明，在所有的可能性，在本文件中报告的属性可以被认为是普遍有效的所有鱼类基因组。(C)2002 Elsevier Science B.V.保留所有权利。
The goal of this study was to provide a comprehensive view of the compositional characteristics of fish genomes. We therefore expanded the number of fish species that we had explored so far in their DNAs by analytical ultracentrifugation in CsCl density gradient from 122 to 201. This study included representatives from three out of nine orders of Elasmobranchs (sharks and rays), both orders of dipnoan lungfishes, and both orders of chondrosteans (sturgeons and bichirs). We also studied 19 out of 38 teleostean orders, which represent all but four (minor) superorders of the subdivision Teleostei, a group comprising about 23,600 species (96% of all extant fishes). This leaves for further studies two subclasses, Holocephali (chimaeras), and Coelacanthimorpha (gombessas). In spite of this substantial increase in the number of species and orders analysed, all average properties (the modal buoyant density, rho(0), the average buoyant density, , the CsCl profile asymmetry, A, and the compositional heterogeneity, H), and all their ranges were unchanged compared to a previous study [J. Mol. Evol. 31 (1990) 265]. This suggests that, in all likelihood, the properties reported in the present paper can be considered as generally valid for all fish genomes. (C) 2002 Elsevier Science B.V. All rights reserved.