Elevated Levels of Pathogenic Indicator Bacteria and Antibiotic Resistance Genes after Hurricane Harvey’s Flooding in Houston
Elevated Levels of Pathogenic Indicator Bacteria and Antibiotic Resistance Genes after Hurricane Harvey’s Flooding in Houston
复制标题
休斯顿飓风哈维引发洪水后致病指示细菌和抗生素抗性基因水平升高
DOI：
10.1021/acs.estlett.8b00329
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发表时间：
2018
影响因子：
10.9
通讯作者：
Stadler, Lauren B.
中科院分区：
文献类型：
--
作者：
Yu, Pingfeng;Zaleski, Avery;Li, Qilin;He, Ya;Mapili, Kris;Pruden, Amy;Alvarez, Pedro J.;Stadler, Lauren B.
城市洪水会极大地影响当地的微生物景观，并增加洪水地区水传播感染的风险。哈维飓风是自2005年卡特里娜飓风以来最具破坏性的飓风，在休斯顿破坏了10万多所房屋，淹没了许多污水处理厂。在这里，我们调查了住宅内外的洪水，河口水，残留河口沉积物立即洪水后收集的微生物群落。水平ofEscherichia coli，一种粪便指示生物，在河口水样中升高，与历史水平相比，人为来源的抗生素耐药性（sul 1/16 S rRNA和intI 1/16 S rRNA）的关键指示基因的相对丰度基于洪水后6个月的监测。定量聚合酶链反应测定结果表明，对应于假定的致病菌的基因标记更丰富的室内洪水比街道洪水和河口水。室内滞水沃茨中16 SrRNA和sul 1基因的丰度也较高。洪水动员的沉积物表现出增加丰富的假定病原体postflood在居民区和公园。总的来说，这项研究表明，极端的洪水可以增加接触病原体和相关风险的水平。
Urban flooding can dramatically affect the local microbial landscape and increase the risk of waterborne infection in flooded areas. Hurricane Harvey, the most destructive hurricane since Katrina in 2005, damaged more than 100000 homes in Houston and flooded numerous wastewater treatment plants. Here we surveyed microbial communities in floodwater inside and outside residences, bayou water, and residual bayou sediment collected immediately postflood. Levels ofEscherichia coli, a fecal indicator organism, were elevated in bayou water samples as compared to historical levels, as were relative abundances of key indicator genes of anthropogenic sources of antibiotic resistance (sul1/16S rRNA andintI1/16S rRNA) based on 6 month postflood monitoring. Quantitative polymerase chain reaction measurements showed that gene markers corresponding to putative pathogenic bacteria were more abundant in indoor floodwater than in street floodwater and bayou water. Higher abundances of 16S rRNA andsul1 genes were also observed in indoor stagnant waters. Sediments mobilized by floodwater exhibited an increased abundance of putative pathogens postflood in both residential areas and public parks. Overall, this study demonstrates that extreme flooding can increase the level of exposure to pathogens and associated risks.