Correlation Between Optical Fluorescence and Microwave Transmission During Single-cell Electroporation
Correlation Between Optical Fluorescence and Microwave Transmission During Single-cell Electroporation
复制标题
单细胞电穿孔过程中光学荧光与微波传输的相关性
DOI：
10.1109/tbme.2018.2885781
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发表时间：
2018
影响因子：
4.6
通讯作者：
Hwang, James C.
中科院分区：
文献类型：
--
作者：
Li, Hang;Ma, Xiao;Du, Xiaotian;Li, Lei;Cheng, Xuanhong;Hwang, James C.
在电穿孔过程中同时通过光学和微波技术对哺乳动物细胞进行多模式表征。方法使用共面波导，在其中心导体的中间捕获Jurkat细胞，施加100 kHz的不同振幅的连续波20 s，每隔0.4s测量一次9GHz的微波透射系数。结果电穿孔开始时，荧光强度和透射系数。此外，在持续300秒的测量中，发现透射系数恢复到预穿孔水平，而荧光强度保持不同。由于细胞被证实是活的，通过后穿孔染色，恢复的透射系数建议可逆electroportion.ConclusionThese实验结果表明，透射系数可以作为一个无标记的指示器的细胞膜通透性在电穿孔过程中和电穿孔后。此外，它可以用来迅速区分可逆的电穿孔从不可逆的one.SignificanceThis研究应该帮助细胞生理学的基本分析，以及分子传递，在细胞工程和电疗。
ObjectiveMultimodal characterization of a mammalian cell by optical and microwave techniques simultaneously during electroporation.MethodsUsing a coplanar waveguide with a Jurkat cell trapped in the middle of its center conductor, continuous waves at 100 kHz of different amplitudes were applied for 20 s, while microwave transmission coefficients at 9 GHz were measured every 0.4 s.ResultsThe onset of electroporation was indicated by abrupt changes in both fluorescence intensity and transmission coefficient. Additionally, in measurements that lasted 300 s, the transmission coefficient was found to recover to the pre-poration level, while the fluorescence intensity remained different. Since the cells were confirmed viable through post-poration staining, the recovery of the transmission coefficient suggested reversible electroporation.ConclusionThese experimental results showed that the transmission coefficient could serve as a label-free indicator of cell membrane permeability during and after electroporation. Furthermore, it could be used to expeditiously differentiate reversible electroporation from the irreversible one.SignificanceThis study should aid fundamental analysis of cell physiology, as well as molecular delivery, in cell engineering and electrotherapy.