Accurate measurement of cholecystokinin in plasma.
Accurate measurement of cholecystokinin in plasma.
复制标题
准确测量血浆中胆囊收缩素。
DOI：
10.1093/clinchem/44.5.991
复制
发表时间：
1998
影响因子：
9.3
通讯作者：
J. Rehfeld
中科院分区：
文献类型：
--
作者：
J. Rehfeld
缺乏可靠的血浆分析方法阻碍了对CCK(CCK)的研究。检测的问题是低血浆浓度，广泛的分子异质性，以及CCK与胃泌素的密切同源性，胃泌素在较高浓度下循环。为了建立准确的CCK RIA，兔、豚鼠和小鼠的抗体滴度从200%到4000000不等。用同源多肽、组织和血浆提取液检测抗血清的特异性。兔92128产生具有足够亲和力(或=10(12)摩尔(-1))的高滴度(-gt；或=500000)抗体和所需的特异性。该抗血清与等摩尔效力的生物活性形式的CCK结合，且与胃泌素无反应。健康人血浆CCK浓度从1.13+/-0.10pmol/L(均值+/-SE，n=26)上升至4.92+/-0.34pmol/L。人血浆的层析显示有微量的CCK-58，主要是CCK-33和CCK-22，以及适量的CCK-8。结果表明，用硫酸盐化的CCK-12类似物制备特异性CCK抗血清是可能的。
Shortage of reliable plasma assays has hampered studies of cholecystokinin (CCK). The assay problems are low plasma concentrations, extensive molecular heterogeneity, and close homology of CCK to gastrin, which circulates in higher concentrations. To develop an accurate CCK RIA, antibodies were raised in rabbits, guinea pigs, and mice in titers from 200 to 4000000. The specificity of the antisera was tested with homologous peptides, and tissue and plasma extracts. Rabbit 92128 produced antibodies in high titer (> or =500000) with sufficient avidity (K(o)eff > or = 10(12) mol(-1)) and the desired specificity. The antiserum binds the bioactive forms of CCK with equimolar potency and displays no reactivity with gastrin. CCK concentrations in plasma from healthy humans rose from 1.13 +/- 0.10 pmol/L (mean +/- SE, n = 26) to 4.92 +/- 0.34 pmol/L after a mixed meal. Chromatography of human plasma revealed traces of CCK-58, a predominance of CCK-33 and CCK-22, and moderate amounts of CCK-8. The results show that it is possible to produce specific CCK-antisera using a sulfated CCK-12 analog.
人血浆中的胆囊收缩素八肽样免疫反应性。
影响因子：
29.4
作者：
Walsh,JH;Lamers,CB;Valenzuela,JE
通讯作者：
Valenzuela,JE
影响因子：
15.9
作者：
LIDDLE, RA;GOLDFINE, ID;WILLIAMS, JA
通讯作者：
WILLIAMS, JA
胆囊收缩素的放射免疫测定。
影响因子：
3.1
作者：
Chang,TM;Chey,WY
通讯作者：
Chey,WY