Isolation and characterization of a unique group of slowly growing mycobacteria: Description of Mycobacterium lentiflavum sp nov
Isolation and characterization of a unique group of slowly growing mycobacteria: Description of Mycobacterium lentiflavum sp nov
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DOI：
10.1128/jcm.34.5.1100-1107.1996
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发表时间：
1996-05-01
影响因子：
9.4
通讯作者：
Bottger, EC
中科院分区：
文献类型：
--
作者：
Springer, B;Wu, WK;Bottger, EC
一组独特的缓慢生长的分枝杆菌被确定的基础上的生长特性，生化和脂质概况，核酸分析。分离株显示生长在22至37摄氏度，黄色色素沉着，吐温80水解，烟酸，硝酸还原酶，尿素酶和阴性测试;芳基硫酸酯酶，吡嗪酰胺酶，热稳定性过氧化氢酶的测试是可变的。细胞脂肪酸的气液色谱和分枝菌酸的薄层色谱和高效液相色谱分析表明，一个独特的模式，这是不同于其他物种。16S rRNA基因序列测定表明，该基因序列与猴分枝杆菌和M.日内瓦。根据DNA同源性研究，我们认为这些生物是一个新物种的代表，其名称M。lentiflavum sp，nov.
A distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. The isolates showed growth at 22 to 37 degrees C, yellow pigmentation, and negative tests for Tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. Analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer chromatography and high-performance liquid chromatography indicated a distinctive pattern which was unlike those of other species. Determination of the 16S rRNA gene sequence showed a unique sequence closely related to Mycobacterium simiae and M. genavense. On the basis of DNA homology studies, we suggest that these organisms are representatives of a novel species, for which the name M. lentiflavum sp, nov. is proposed.