Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay
Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay
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DOI：
10.1016/j.colsurfb.2010.03.012
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发表时间：
2010-07-01
影响因子：
5.8
通讯作者：
Huo, Qun
中科院分区：
文献类型：
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作者：
Huo, Qun
蛋白质-蛋白质相互作用和蛋白质复合物/聚集体的形成在几乎所有的生物功能和活动中起着至关重要的作用。通过纳米颗粒聚集免疫测定，我们发现一些蛋白质在癌组织中比正常组织中更复杂/聚集。本研究检测了卵巢、结肠和前列腺组织裂解物中的四种生物标志物蛋白，CA 125、CEA（癌胚抗原）、CA 19 -9和PAP（前列腺酸性磷酸酶）。从前列腺组织的PAP测定中观察到最令人兴奋的结果：基于前列腺组织裂解物中PAP的复合物/聚集水平，可以将前列腺癌与正常前列腺和具有良性病症如BPH（良性前列腺增生）的前列腺清楚地区分。蛋白质的复合物/聚集体水平可以是用于癌症检测和诊断的潜在生物标志物。(C)2010 Elsevier B. V.保留所有权利。
Protein-protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis. (C) 2010 Elsevier B.V. All rights reserved.