Inhibition of the Exocyst Complex with Endosidin 2 Reduces Polarized Growth in Physcomitrium patens.
Inhibition of the Exocyst Complex with Endosidin 2 Reduces Polarized Growth in Physcomitrium patens.
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DOI：
10.17912/micropub.biology.000655
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发表时间：
2022
影响因子：
--
通讯作者：
Vidali, Luis
中科院分区：
文献类型：
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作者：
Bormann, Eric;Xu, Rholee;Nargi, Clare;Wu, Min;Vidali, Luis
内皮素2（ES2）是一种细胞渗透性药物，可与胞吐复合物的Exo70亚基结合，破坏胞吐的最后阶段。这允许对胞吐过程进行剂量依赖性控制，并且更容易研究胞吐依赖性过程，例如极化细胞生长。ES2被用于研究苔藓立碗藓的极化细胞生长，其中植物暴露于浓度增加的ES2，IC50在8.8和12.3 µM之间。在50 μM时，尖端生长的细胞在其尖端附近破裂，这表明ES2通过胞吐作用抑制新细胞壁物质的沉积。该数据用于进一步支持使用ES2作为干扰胞吐作用的工具，仅在高水平的ES2下观察到致死性。
Endosidin 2 (ES2) is a cell-permeable drug that binds to the Exo70 subunit of the exocyst complex, disrupting the final stages of exocytosis. This allows for a dose-dependent control over the process of exocytosis and greater ease in studying exocytic-dependent processes such as polarized cell growth. ES2 was utilized in studying polarized cell growth in the moss Physcomitrium patens , in which plants were exposed to increasing concentrations of ES2 with an IC50 between 8.8 and 12.3 µM. At 50 µM, tip-growing cells ruptured close to their tips, an indication that ES2 inhibits the deposition of new cell wall material via exocytosis. This data serves to further support the use of ES2 as a tool to interfere with exocytosis with lethality only seen at high levels of ES2.