Technical notes for age estimation using the femur: influence of various analytical conditions on D-aspartic acid contents
Technical notes for age estimation using the femur: influence of various analytical conditions on D-aspartic acid contents
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使用股骨进行年龄估算的技术说明：各种分析条件对 D-天冬氨酸含量的影响
DOI：
10.1007/s00414-002-0339-2
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发表时间：
2002
影响因子：
2.1
通讯作者：
S. Ohtani
中科院分区：
文献类型：
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作者：
S. Ohtani
为了使一个更好的估计个人的年龄使用骨样本，我们评估了各种标准的影响，分析测量的D-天冬氨酸含量。使用来自男性股骨的密质骨，我们改变了六种分析条件（样品体积、样品粒度、水解温度、水解时间、水解期间的盐酸体积和水解期间的盐酸浓度）。D型/L型比例受水解温度影响最大（估计年龄差异为3.03年/°C），其次是盐酸体积（1.44年/ml）和盐酸浓度（0.69年/0.1 M）。较大的样品粒度和水解过程中的盐酸体积往往会导致较低的外消旋速率。但在5-50 mg范围内，取样量对D-天冬氨酸含量的测定无影响。由于股骨密质骨的外消旋化反应速率比牙本质慢，因此骨样品似乎比牙本质更受分析条件的影响。因此，在使用股骨样本估计年龄时，必须谨慎进行试验，特别是关于水解温度、盐酸体积和浓度。
In order to make a better estimate of the individual age using bone samples, we evaluated the effects of various criteria on the analytical measurement of D-aspartic acid contents. Using compact bone from a male femur, we varied six analytical conditions (sample volume, sample particle size, hydrolysis temperature, hydrolysis time, hydrochloric acid volume during hydrolysis, and hydrochloric acid concentration during hydrolysis). D-form/L-form ratios were affected most by hydrolysis temperature (estimated age differences were 3.03 years/°C), followed in order by hydrochloric acid volume (1.44 years/ml) and hydrochloric acid concentration (0.69 years/0.1 M). Larger sample particle sizes and hydrochloric acid volumes during hydrolysis tended to result in lower racemization rates. Nonetheless, within a range of 5–50 mg, sampling volume did not affect the detected D-aspartic acid contents. Since the racemization reaction rate in femur compact bone is slower than in dentin, bone samples seem to be more greatly influenced by analytic conditions than dentin. Tests must therefore be performed with caution, especially with regard to the hydrolysis temperature, hydrochloric acid volume and concentration when estimating age using femur samples.
根据牙齿中氨基酸的外消旋作用估算年龄
DOI：
--
发表时间：
2004
期刊：
影响因子：
--
作者：
Ohtani S;Ito R;Arany S;Yamamoto T;大谷 進;Susumu Ohtani;Susumu Ohtani;Szilvia Arany;Susumu Ohtani;Szilvia Arany;Katsuichi Yamamoto;Susumu Ohtani;Szilvia Arany;大谷 進;大谷 進;Susumu Ohtani;大谷 進;大谷 進;大谷 進;Susumu Ohtani;大谷 進;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani;大谷 進;Susum Ohtani
通讯作者：
Susum Ohtani