Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.
Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.
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小鼠腹腔巨噬细胞响应吞噬刺激释放白三烯 C。
DOI：
10.1073/pnas.77.8.4928
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发表时间：
1980
影响因子：
11.1
通讯作者：
Manning,JM
中科院分区：
文献类型：
--
作者：
Rouzer,CA;Scott,WA;Cohn,ZA;Blackburn,P;Manning,JM
在豚鼠回肠试验中，摄入酵母聚糖颗粒的小鼠腹腔巨噬细胞释放出具有慢反应物质活性的花生四烯酸极性代谢物。经溶剂萃取、Sephadex G-25柱层析纯化代谢产物。纯化的代谢产物在280 nm处吸收光，并含有游离氨基。当巨噬细胞与[3 H]花生四烯酸、[35 H]半胱氨酸或[14 C]谷氨酸预孵育过夜时，每种放射性标记均掺入化合物中。直接氨基酸分析显示甘氨酸、谷氨酸和半胱氨酸的摩尔比为0.97：1.00：0.82。上述数据与花生四烯酸和谷胱甘肽的加合物白三烯C的结构一致。基于[3 H]花生四烯酸掺入或氨基酸分析的白三烯C定量表明，在最大吞噬刺激后，6 × 10（7）巨噬细胞（3.6 mg细胞蛋白）释放7.5 nmol。纯化的白三烯C具有11，500单位/nmol的慢反应物质活性（在豚鼠回肠收缩试验中，1单位具有5 ng组胺的活性）。
Mouse peritoneal macrophages that had ingested zymosan particles released a polar metabolite of arachidonic acid possessing slow-reacting substance activity in the guinea pig ileum assay. The metabolite was purified by solvent extraction, Sephadex G-25 column chromatography. The purified metabolite absorbed light at 280 nm and contained a free amino group. When macrophages were preincubated overnight with [3H]arachidonic acid, [35H]cysteine, or [14C]glutamic acid, each radiolabel was incorporated into the compound. Direct amino acid analysis revealed glycine, glutamic acid, and cysteine at molar ratios of 0.97:1.00:0.82. The above data were consistent with the structure of leukotriene C, an adduct of arachidonic acid and glutaathione. Quantification of the leukotriene C based on incorporation of [3H]arachidonic acid or amino acid analysis indicated that 6 X 10(7) macrophages (3.6 mg of cell protein) released 7.5 nmol after a maximal phagocytic stimulus. The purified leukotriene C had a slow reacting substance activity of 11,500 units/nmol (1 unit has the activity of 5 ng of histamine in a guinea pig ileum contraction assay).
容易感染的医院病人
影响因子：
17.3
作者：
A. Pollock
通讯作者：
A. Pollock