Pentoxifylline exerts anti-inflammatory effects on cerebral ischemia reperfusion-induced injury in a rat model via the p38 mitogen-activated protein kinase signaling pathway
Pentoxifylline exerts anti-inflammatory effects on cerebral ischemia reperfusion-induced injury in a rat model via the p38 mitogen-activated protein kinase signaling pathway
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DOI：
10.3892/mmr.2017.7953
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发表时间：
2018-01-01
影响因子：
3.4
通讯作者：
Xie, Weiwei
中科院分区：
文献类型：
--
作者：
Dong, Jianqing;Yuan, Xinmei;Xie, Weiwei
喷替福林具有复杂的功能和广泛的药理作用，由于其治疗效果和在体内的快速代谢而用于治疗，没有累积作用，副作用少。本研究观察了戊茶碱通过抑制炎症反应对大鼠脑缺血再灌注损伤的影响。苏木精-伊红染色观察神经细胞数量，酶联免疫吸附法测定肿瘤坏死因子、白细胞介素-6、丙二醛和超氧化物歧化酶活性。喷替福林治疗可显著恢复脑缺血再灌注大鼠神经功能缺损评分和脑梗死体积。此外，戊茶碱治疗显着逆转脑缺血再灌注诱导的白细胞介素6，肿瘤坏死因子，丙二醛和超氧化物歧化酶水平在体内。此外，喷替福林显着抑制环氧合酶-2和诱导型一氧化氮合酶的mRNA和蛋白质表达在脑IRI小鼠。用戊氨酰茶碱治疗还显著抑制脑IRI小鼠中裂解的caspase-3和p38丝裂原活化蛋白激酶（MAPK）蛋白的表达。这些结果表明，戊茶碱对脑IRI的保护作用可能通过p38 MAPK信号通路发生。
Pentoxifylline exhibits complex functions with extensive pharmacological effects and is used therapeutically due to its therapeutic effects and rapid metabolism in the body, with no cumulative effects and few side effects. The present study investigated the effects of pentoxifylline on cerebral ischemia reperfusion-induced injury (IRI) through suppression of inflammation in rats. Hematoxylin and eosin staining was performed to evaluate the number of neurocytes, and ELISAs were applied to measure tumor necrosis factor-, interleukin-6, malondialdehyde and superoxide dismutase activities. Treatment with pentoxifylline significantly recovered the cerebral ischemia reperfusion-induced neurological deficit score and cerebral infarct volume in rats. In addition, pentoxifylline treatment significantly reversed the cerebral ischemia reperfusion-induced interleukin-6, tumor necrosis factor-, malondialdehyde and superoxide dismutase levels in vivo. Furthermore, pentoxifylline significantly inhibited cyclooxygenase-2 and inducible nitric oxide synthase mRNA and protein expression in cerebral IRI mice. Treatment with pentoxifylline also significantly suppressed the expression of cleaved caspase-3 and p38 mitogen-activated protein kinase (MAPK) protein in cerebral IRI mice. These results indicate that the protective effects of pentoxifylline on cerebral IRI may occur via the p38 MAPK signaling pathway.