A mouse model for acute otitis media
A mouse model for acute otitis media
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DOI：
10.1034/j.1600-0463.2003.1111012.x
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发表时间：
2003-10-01
期刊：
影响因子：
2.8
通讯作者：
Ryan, AF
中科院分区：
文献类型：
--
作者：
Melhus, Å;Ryan, AF
为了诱导小鼠中的急性中耳炎并描述感染的临床和细菌学病程，BALB/C，瑞士 - 伍斯特和C57BL/6小鼠的中耳被肺炎链球菌，嗜血杆菌和摩西氏菌Catarrhalis接触。通过临床观察，耳鼻镜检查以及对血液和中耳细菌样品的分析来监测系统性和局部变化。还测试了Cartarrhalis捕集小鼠红细胞的凝集。根据细菌菌株，细菌剂量和小鼠菌株的不同，三个反应：急性中耳炎，浆液积液的中耳炎或无反应。 BALB/C小鼠是最易感的动物。在第3天，有76％的BALB/C小鼠发生了中耳感染，50％的中耳培养物阳性，56％为细菌，而10％屈服于传播的感染。当地感染持续了大约一周。幸存下来的动物恢复而没有永久性恶化或耳鼻镜可见的变化。在任何情况下，卡他氏菌在任何情况下都不会引起培养阳性的中耳感染，这可能是由于无法凝集小鼠红细胞。小鼠模型可以成为研究肺炎球菌和流感h.诱导的中耳炎培养基的有用工具，但是必须仔细滴定细菌剂量并将其调整为所选小鼠菌株。
To induce acute otitis media in the mouse and to describe the clinical and bacteriological course of the infection, middle ears of BALB/c, Swiss-Webster and C57BL/6 mice were inoculated with Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis. Systemic and local changes were monitored by clinical observations, otomicroscopy, and analysis of bacterial samples from blood and middle ears. Agglutination of mouse erythrocytes by M. catarrhalis was also tested. Depending on bacterial strain, bacterial dose, and mouse strain three responses were identified: acute otitis media, otitis media with serous effusion, or no reaction. BALB/c mice were the most susceptible animals. On day 3, 76% of the BALB/c mice had developed middle ear infection, 50% had a positive middle ear culture, 56% were bacteremic, and 10% had succumbed to a disseminated infection. The local infections lasted approximately a week. Animals which survived recovered without permanent deterioration or otomicroscopically discernible changes. In no case did M. catarrhalis induce a culture-positive middle ear infection, possibly due to an inability to agglutinate the mouse erythrocytes. The mouse model can become a useful tool in studies of pneumococcal and H. influenzae-induced otitis media, but the bacterial dose has to be carefully titrated and adjusted to the chosen mouse strain.