Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana
Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana
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DOI：
10.1007/s00438-002-0709-y
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发表时间：
2002-08-01
影响因子：
3.1
通讯作者：
Bohlmann, J
中科院分区：
文献类型：
--
作者：
Aubourg, S;Lecharny, A;Bohlmann, J
通过拟南芥中的基因组序列分析发现了一个40个萜类合酶基因（ATTP）的家族。这是目前以任何物种而闻名的最大，最多样化的TPS基因。在TPS基因中，将植物TPS超家族的五个系统发育亚家族聚集。令人惊讶的是，在基因结构，序列相关性和系统发育位置的各个方面，植物单苯乙酸合酶的基因，倍苯二酚合成酶或二甲酸酯合成酶的二次代谢基因都非常相似。这些ATTP的快速演变是由于基因结构的重复基因复制和序列差异而产生的。相比之下，只有两个ATTP基因在碱性（原发性）代谢中具有已知功能，即吉布雷素生物合成。原发性和次要代谢的基因多样化速率的显着差异与理解萜类自然产物多样性的演变有关。八个ATTPS基因被中断，可能是无活跃的假基因。 TPS基因在所有五个染色体上的定位都反映了拟南芥基因组的动力学。但是，几个attps基因被聚集并在串联重复序列中组织。此外。某些ATTPS基因与连续的基因组簇中的前烯基转移酶基因（ATGGPPS，香烷基双磷酸合酶）定位，该基因组簇编码了萜类化合物合成中的连续步骤。聚类的组织可能对TPS基因的演化有影响，并且途径段的演化是萜类自然产物的合成。系统发育分析重点介绍了TPS旁系同源物差异的事件，并暗示了直系同源基因和TPS基因家族进化的模型。
A family of 40 terpenoid synthase genes (AtTPS) was discovered by genome sequence analysis in Arabidopsis thaliana. This is the largest and most diverse group of TPS genes currently known for any species. At TPS genes cluster into five phylogenetic subfamilies of the plant TPS superfamily. Surprisingly, thirty AtTPS closely resemble, in all aspects of gene architecture, sequence relatedness and phylogenetic placement, the genes for plant monoterpene synthases, sesquiterpene synthases or diterpene synthases of secondary metabolism. Rapid evolution of these AtTPS resulted from repeated gene duplication and sequence divergence with minor changes in gene architecture. In contrast, only two AtTPS genes have known functions in basic (primary) metabolism, namely gibberellin biosynthesis. This striking difference in rates of gene diversification in primary and secondary metabolism is relevant for an understanding of the evolution of terpenoid natural product diversity. Eight AtTPS genes are interrupted and are likely to be inactive pseudogenes. The localization of At TPS genes on all five chromosomes reflects the dynamics of the Arabidopsis genome; however, several AtTPS genes are Clustered and organized in tandem repeats. Furthermore. some AtTPS genes are localized with prenyltransferase genes (AtGGPPS, geranylgeranyl diphosphate synthase) in contiguous genomic clusters encoding consecutive steps in terpenoid biosynthesis. The clustered organization may have implications for TPS gene evolution and the evolution Of pathway segments for the synthesis of terpenoid natural products. Phylogenetic analyses highlight events in the divergence of the TPS paralogs and suggest orthologous genes and a model for the evolution of the TPS gene family.