Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets
Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets
复制标题
转录组分析揭示黑磷纳米片诱导肠病性大肠杆菌生长的机制
DOI：
10.1021/acsnano.2c09964
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发表时间：
2023-01-05
期刊：
影响因子：
17.1
通讯作者：
Xing，Baoshan
中科院分区：
文献类型：
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作者：
Xiong，Zhiqiang;Zhang，Xuejiao;Xing，Baoshan
随着黑磷（BP）纳米片的广泛生产和应用，其向环境释放是不可避免的，这引起了人们对这种二维（2D）材料对环境微生物等敏感受体的命运和影响的关注。虽然BP纳米片的细菌毒性已被证实，但生物反应在病原微生物菌株和非病原微生物菌株中是否不同尚不清楚。这里，肠致病性大肠杆菌（EPEC）和非致病性大肠杆菌h5α (E. coliDH5α)，大肠杆菌12 （E. colik12）和热带芽孢杆菌(B。对BP纳米片的微生物毒性进行了比较研究。在10至100 μg ml - 1的剂量范围内暴露于BP纳米片12小时后，EPEC经历了增强的生长和e。coliDH5α安德。12 . b。热带菌表现出明显的毒性。通过结合转录组测序、蛋白质组分析等敏感生物学技术，揭示了bp诱导EPEC促进生长的机制。简而言之，BP纳米片激活抗氧化系统，抵抗氧化应激，促进蛋白质合成和分泌，减轻膜损伤，增强能量供应，激活生长相关途径。这些影响在非致病性菌株中都不明显。通过描述菌株依赖的微生物效应机制，本研究不仅强调了BP纳米片对环境和人类健康的潜在风险，而且还提醒人们注意在评估新兴纳米材料的危害和毒性时选择模型菌株的重要性。
With the extensive production and application of black phosphorus (BP) nanosheets, release to the environment is inevitable, which raises concerns about the fate and effects of this two-dimensional (2D) material on sensitive receptors such as environmental microbes. Although the bacterial toxicity of BP nanosheets has been demonstrated, whether the biological response differs in pathogenic and nonpathogenic strains of a microorganism is unknown. Here, enteropathogenicEscherichia coli(EPEC) and nonpathogenicEscherichia coliDH5α (E. coliDH5α),Escherichia colik12 (E. colik12), andBacillus tropicus(B. tropicus) are used to comparatively study the microbial toxicity of BP nanosheets. Upon exposure to BP nanosheets across a range of doses from 10 to 100 μg mL–1for 12 h, EPEC experienced enhanced growth andE. coliDH5α andE. colik12 were not affected, whereasB. tropicusexhibited clear toxicity. By combining transcriptome sequencing, proteome analysis, and other sensitive biological techniques, the mechanism of BP-induced growth promotion for EPEC was uncovered. Briefly, BP nanosheets activate the antioxidation system to resist oxidative stress, promote protein synthesis and secretion to attenuate membrane damage, enhance the energy supply, and activate growth-related pathways. None of these impacts were evident with nonpathogenic strains. By describing the mechanism of strain-dependent microbial effects, this study not only highlights the potential risks of BP nanosheets to the environment and to human health but also calls attention to the importance of model strain selection when evaluating the hazard and toxicity of emerging nanomaterials.