Volumetric and Angiogenic Evaluation of Antitumor Effects with Acoustic Liposome and High-Frequency Ultrasound
Volumetric and Angiogenic Evaluation of Antitumor Effects with Acoustic Liposome and High-Frequency Ultrasound
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DOI：
10.1158/0008-5472.can-11-2389
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发表时间：
2011-11-15
期刊：
影响因子：
11.2
通讯作者：
Mori, Shiro
中科院分区：
文献类型：
--
作者：
Kodama, Tetsuya;Tomita, Noriko;Mori, Shiro
声学脂质体（AL）具有其固有的回声性，并可作为具有组织特异性的药物载体。通过高频超声（HF-US）成像对AL的血管内运动轨迹进行成像，评价不均匀的血管结构和血管分支/弯曲。然而，通过AL和HF-US成像评价抗肿瘤作用对血管生成的影响尚未报道。在这里，我们表明，ALs和高频超声成像系统的组合能够在各种癌症的临床前小鼠模型中非侵入性地评估抗肿瘤体积和血管生成作用。在这项研究中，顺铂对荷两种不同类型肿瘤细胞的小鼠的肿瘤生长和血管生成的抗肿瘤作用进行了评估。通过跟踪血管中的每一个AL流动并将图像传输到个人计算机，利用基于SD方法的色差映射和重建微血管结构。体内生物发光成像系统和免疫组织化学分析证实了抗肿瘤作用。我们的研究结果表明，顺铂通过减少肿瘤内血管面积来抑制肿瘤生长，但不影响肿瘤中的血管生成率。肿瘤外区的血管占有率大于内区，但两者均与对照肿瘤相似。我们建议，这种方法映射微血管与AL和HF-US系统可以作为一种新的分子成像方法，用于评估血管生成，并可应用于评估各种治疗药物的抗肿瘤效果。Cancer Res; 71（22）; 6957-64.（C）2011年《非洲标准化评论》。
Acoustic liposomes (AL) have their inherent echogenicity and can add functionality in serving as drug carriers with tissue specificity. Nonuniform vascular structures and vascular branches/bends are evaluated by imaging the intravascular movement locus of ALs with high-frequency ultrasound (HF-US) imaging. However, the evaluation of antitumor effects on angiogenesis by ALs and HF-US imaging has not been reported. Here, we show that the combination of ALs and an HF-US imaging system is capable of noninvasively evaluating antitumor volumetric and angiogenic effects in preclinical mouse models of various cancers. In this study, the antitumor effects of cisplatin on tumor growth and angiogenesis in mice bearing two different types of tumor cells were assessed. By tracking each AL flowing in the vessel and transferring the images to personal computers, microvessel structures were mapped and reconstructed using the color difference based on SD method. The antitumor effects were confirmed with an in vivo bioluminescence imaging system and immunohistochemical analysis. Our results show that cisplatin inhibits tumor growth by decreasing intratumoral vessel area but does not affect the angiogenesis ratio in the tumor. The vascular occupancy in the outer region of the tumor was larger than that in the inner region; however, both occupancies were similar to those of the control tumor. We propose that this method of mapping microvessels with ALs and an HF-US system can serve as a new molecular imaging method for the assessment of angiogenesis and can be applied to evaluate the antitumor effects by various therapeutic agents. Cancer Res; 71(22); 6957-64. (C)2011 AACR.