High-dose dietary exposure of mice to perfluorooctanoate or perfluorooctane sulfonate exerts toxic effects on myeloid and B-lymphoid cells in the bone marrow and these effects are partially dependent on reduced food Consumption
High-dose dietary exposure of mice to perfluorooctanoate or perfluorooctane sulfonate exerts toxic effects on myeloid and B-lymphoid cells in the bone marrow and these effects are partially dependent on reduced food Consumption
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DOI：
10.1016/j.fct.2012.06.023
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发表时间：
2012-09-01
影响因子：
4.3
通讯作者：
Abedi-Valugerdi, Manuchehr
中科院分区：
文献类型：
--
作者：
Qazi, Mousumi Rahman;Nelson, B. Dean;Abedi-Valugerdi, Manuchehr
众所周知，小鼠接触全氟辛酸盐（PFOA）或全氟辛烷磺酸（PFOS）会对胸腺和脾脏产生不利影响。在这里，我们描述了使用这些化合物（0.001-0.02%，w/w）进行为期 10 天的饮食治疗对小鼠骨髓 (BM) 的影响。当剂量为 0.02% 时，两种化合物都会减少食物消耗并导致胸腺和脾脏萎缩。在相同剂量下，组织病理学和流式细胞术分析显示：（i）BM 总数以及髓系细胞、pro/pre B 细胞、未成熟 B 细胞和早期成熟 B 细胞的数量均显着减少； (ii) 这些副作用在停用这些化合物后 10 天部分或完全逆转。在 0.002% 的较低剂量下，仅 PFOA 减少了 B 淋巴细胞数量。最后，给小鼠喂食相当于暴露于 0.02% PFOA 的动物消耗量的饮食，也表现出胸腺和脾脏萎缩，以及 B 淋巴细胞数量减少，但不影响骨髓细胞。因此，在小鼠中，免疫毒性剂量的 PFOA 或 PFOS 会对骨髓中的骨髓细胞和 B 淋巴细胞产生不利影响，部分原因是食物消耗减少。 (C) 2012 Elsevier Ltd. 保留所有权利。
It is well established that exposure of mice to perfluorooctanoate (PFOA) or perfluorooctane sulfonate (PFOS) exerts adverse effects on the thymus and spleen. Here, we characterize the effects of a 10-day dietary treatment with these compounds (0.001-0.02%, w/w) on the bone marrow (BM) of mice. At a dose of 0.02%, both compounds reduced food consumption and caused atrophy of the thymus and spleen. At this same dose, histopathological and flow cytometric analysis revealed that (i) the total numbers of BM as well as the numbers of myeloid, pro/pre B, immature B and early mature B cells were all reduced significantly; and (ii) these adverse effects were reversed either partially or completely 10 days after withdrawal of these compounds. At the lower dose of 0.002%, only PFOA reduced the B-lymphoid cell population. Finally, mice fed an amount of diet equivalent to that consumed by the animals exposed to 0.02% PFOA also exhibited atrophy of the thymus and spleen, and a reduction in the number of B-lymphoid population, without affecting myeloid cells. Thus, in mice, immunotoxic doses of PFOA or PFOS induce adverse effects on the myeloid and B-lymphoid cells in the BM, in part as a consequence of reduced food consumption. (C) 2012 Elsevier Ltd. All rights reserved.