Genome-wide association study (GWAS) delineates genomic loci for ten nutritional elements in foxtail millet (Setaria italica L.)
Genome-wide association study (GWAS) delineates genomic loci for ten nutritional elements in foxtail millet (Setaria italica L.)
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DOI：
10.1016/j.jcs.2018.11.006
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发表时间：
2019-01-01
影响因子：
3.8
通讯作者：
Prasad, Manoj
中科院分区：
文献类型：
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作者：
Jaiswanl, Vandana;Bandyopadhyay, Tirthankar;Prasad, Manoj
营养缺乏被认为是对人类健康的一个主要威胁，特别是在低收入国家。因此，必须提高重要粮食作物的营养品质。小米是第二大种植小米和2-5倍的营养丰富的主要谷类作物。在本研究中，我们确定了10个营养元素的遗传决定因素，包括钾，镍，钙，硼，镁，磷，硫，锌，锰和铁的第一次在谷子。为此目的，全基因组关联研究（GWAS）进行了93个不同的加入和10 K SNP（分布在所有9个谷子染色体）。共鉴定出74个与上述10种元素相关的标记-性状关联（MTAs），其中10个（与B、Mg、Zn和Fe相关）具有较高的置信度[-log（p）> 5.78]。确定理想的SNP等位基因和有利的单倍型可能证明在狗尾草育种有用。此外，显著的协同效应表明，通过组合一种以上的MTA，可以显著增强相关元素。可以选择位于关联信号内或附近的候选基因用于功能表征。所鉴定的上级基因型可作为谷子分子标记辅助育种的潜在供体。
Nutritional deficiency is found to be a major threat to human health, especially in low-income countries. Thus it is essential to improve nutritional qualities of important food crops. Foxtail millet is second largest cultivated millet and 2-5 times nutritionally richer than major cereal crops. During the present study, we identified genetic determinants of ten nutritional elements including potassium, nickel, calcium, boron, magnesium, phosphorus, sulphur, zinc, manganese and iron for the first time in foxtail millet. For this purpose, genome-wide association studies (GWAS) were conducted using 93 diverse accessions and 10 K SNPs (distributed across all the nine foxtail millet chromosomes). Altogether, 74 marker-trait associations (MTAs) were identified to be associated with above mentioned ten elements, out of which ten (10) MTAs (associated with B, Mg, Zn and Fe) showed high confidence [-log(p) > 5.78]. Identified desirable SNP alleles and favourable haplotypes may prove useful in foxtail breeding. Also, significant pyramiding effect suggested that associated elements can be substantially enhanced through combining more than one MTA. Candidate genes residing within or near the association signal may be selected for functional characterization. Superior genotypes identified may prove as a potential donor in foxtail millet breeding assisted through the molecular marker.