MOESIN - A CELL-MEMBRANE PROTEIN LINKED WITH SUSCEPTIBILITY TO MEASLES-VIRUS INFECTION
MOESIN - A CELL-MEMBRANE PROTEIN LINKED WITH SUSCEPTIBILITY TO MEASLES-VIRUS INFECTION
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DOI：
10.1006/viro.1994.1029
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发表时间：
1994-01-01
期刊：
影响因子：
3.7
通讯作者：
TERMEULEN, V
中科院分区：
文献类型：
--
作者：
DUNSTER, LM;SCHNEIDERSCHAULIES, J;TERMEULEN, V
麻疹病毒是一种高度传染性的病毒，可引起人类急性和持续性疾病，其受体仍不清楚。我们分离出了一种单克隆抗体（mAb），命名为 mAb 119，它以剂量依赖性方式特异性抑制麻疹病毒对易感细胞系的感染。该抗体从 125I 表面标记的细胞中沉淀出表观分子量为 75 kDa 的蛋白质，其表位存在于人外周血单核细胞、人类细胞系和非洲绿猴细胞系 Vero 上。使用共价结合的 mAb 119 在 Sepharose 柱上对去污剂溶解的细胞膜蛋白进行亲和层析，从而部分纯化 75-kDa 蛋白。麻疹病毒与这种亲和纯化的蛋白质预孵育可剂量依赖性地抑制麻疹病毒感染。该蛋白质的氨基酸微测序揭示了其与人膜组织延伸刺突蛋白moesin的同一性，moesin是一种与细胞质膜相关的细胞内和细胞外蛋白质。随后，还发现针对纯化的 moesin (mAb 38/87) 产生的抗体可以特异性抑制易感细胞的麻疹病毒感染，并证实了我们使用 mAb 119 获得的数据。我们的数据表明，moesin 充当麻疹病毒的受体。
Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.