Increased levels of luminol-enhanced chemiluminescence by rectal mucosa of patients with colonic neoplasia: a possible marker for colonic neoplasia.
Increased levels of luminol-enhanced chemiluminescence by rectal mucosa of patients with colonic neoplasia: a possible marker for colonic neoplasia.
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结肠瘤形成患者直肠粘膜的鲁米诺增强化学发光水平增加：结肠瘤形成的可能标志物。
DOI：
10.1080/01635589309514250
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Mobarhan,S
中科院分区：
文献类型：
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作者：
Keshavarzian,A;Olyaee,M;Sontag,S;Mobarhan,S
结肠肿瘤患者的结肠细胞增殖（CCP）增加。先前在大鼠中的研究表明，CCP增加与活性氧代谢产物（ROM）产生增加密切相关。我们假设，在人类中，ROM的产生也参与了CCP的增加。使用化学发光探针，我们估计了37例患者的直肠粘膜中的ROM的水平，这些患者以前有结肠肿瘤（14例癌症和23例息肉）和20例对照组，这些对照组结肠镜检查正常，没有结肠肿瘤病史。与对照受试者的直肠粘膜相比，结肠肿瘤患者的正常直肠粘膜含有显著更高水平的鲁米诺增强化学发光（LECL）（p < 0.01）。息肉患者和癌症患者的LECL水平没有差异。20名对照中的4名和37名患者中的31名的LECL水平> 1，000 cpm/mg蛋白（阳性和阴性预测值分别为89%和73%）。吲哚美辛，一种特异性的环氧合酶抑制剂，加入到组织悬液中显著降低LECL水平。这些初步数据表明，1）ROM可能参与CCP的发生率和恶性细胞转化过程，2）花生四烯酸通过环氧合酶途径氧化显著促进直肠粘膜中ROM的产生，3）测量直肠粘膜产生的LECL水平可能是筛查结肠肿瘤的敏感标志物。
Increased colonic cell proliferation (CCP) has been reported in patients with colonic neoplasia. Previous studies in rats suggest that increased CCP is closely related to increased reactive oxygen metabolite (ROM) production. We hypothesized that, in humans, ROM production is also involved in increased CCP. Using a chemiluminescence probe, we estimated the levels of ROMs in the rectal mucosa of 37 patients who previously had colonic neoplasia (14 with cancer and 23 with polyps) and 20 control subjects who had normal colonoscopic examination and no history of colonic neoplasia. Normal‐appearing rectal mucosa of patients with colonic neoplasia contained significantly higher levels of luminol‐enhanced chemiluminescence (LECL) than rectal mucosa of control subjects (p < 0.01). There was no difference in LECL levels between patients with polyps and patients with cancer. Four of 20 controls and 31 of 37 patients had LECL levels 1,000 cpm/mg protein (positive and negative predictive values of 89% and 73%, respectively). Addition of indomethacin, a specific cyclooxygenase inhibitor, to the tissue suspension significantly decreased LECL levels. These preliminary data suggest that 1) ROMs may be involved in both the rate of CCP and the process of malignant cellular transformation, 2) oxidation of arachidonic acid via the cyclooxygenase pathway significantly contributes to the production of ROMs in rectal mucosa, and 3) measurement of the levels of LECL produced by the rectal mucosa may be a sensitive marker to screen for colonic neoplasia.