The influence of swelling and matrix degradation on the microstructural integrity of tendon
The influence of swelling and matrix degradation on the microstructural integrity of tendon
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DOI：
10.1016/j.actbio.2006.05.008
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发表时间：
2006-09-01
影响因子：
9.7
通讯作者：
Shelton, Julia C.
中科院分区：
文献类型：
--
作者：
Screen, Hazel R. C.;Chhaya, Vivek H.;Shelton, Julia C.
肌腱是一种多层次的纤维复合材料，负责将肌肉的力量传递到骨骼。它由富含蛋白聚糖的基质包围的胶原单位的分级排列组成，排列以支持应变传递，从而有助于肌腱的机械行为。本研究探讨了肿胀和酶降解对肌腱层次结构完整性的影响。在对样品完整性进行机械表征之前，使用生物化学和微观结构分析来检查孵育对基质的组成和溶胀的影响。结果表明，肌腱原纤维和原纤维间基质显着肿胀后，在磷酸盐缓冲盐水中孵育，导致极限拉伸载荷的减少，与失败之间的原纤维和子原纤维开始。相反，与软骨素酶ABC孵育导致从样品中完全去除糖胺聚糖，随后减少肿胀的程度。这些纤维束也表现出失效载荷的增加，失效主要发生在纤维之间。这项工作的结果证实了非胶原基质成分在控制肌腱结构内的应变传递中的重要性。它还强调了在测试之前将样品保持在适当和受控环境中的必要性。(C)2006 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Tendon is multi-level fibre composite material, responsible for the transmission of forces from muscles to the skeleton. It is composed of a hierarchical arrangement of collagenous units surrounded by a proteoglycan-rich matrix, arranged to support strain transfer, and thus contribute to the mechanical behaviour of tendon. This study examines the effect of swelling and enzymatic degradation on structural integrity at different levels of the tendon hierarchy. Biochemical and microstructural analysis are used to examine the effects of incubation on the composition and swelling of the matrix, prior to a mechanical characterisation of sample integrity. Results indicated significant swelling of tendon fibrils and interfibrillar matrix after incubation in phosphate buffered saline, leading to a reduction in ultimate tensile load, with failure initiated between fibrils and sub-fibrils. In contrast, incubation with the enzyme chondroitinase ABC resulted in a total removal of glycosaminoglycan from the samples, and a subsequent reduction in the extent of swelling. These fascicles also demonstrated an increase in failure loads, with failure predominating between fibres. The findings from this work confirm the importance of the non-collagenous matrix components in controlling strain transfer within tendon structures. It also highlights the necessity to maintain samples within a suitable and controlled environment prior to testing. (C) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.