Generic injuries are sufficient to induce ectopic Wnt organizers in Hydra.
Generic injuries are sufficient to induce ectopic Wnt organizers in Hydra.
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DOI：
10.7554/elife.60562
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发表时间：
2021-03-29
期刊：
影响因子：
7.7
通讯作者：
Juliano CE
中科院分区：
文献类型：
--
作者：
Cazet JF;Cho A;Juliano CE
在全身再生过程中，二分损伤可以引发两种不同类型的再生。为了了解这种适应性反应背后的转录调控，我们表征了刺胞动物九头蛇口腔和口体再生过程中的转录丰度和染色质可及性。我们发现两个伤口部位对截肢的初始反应是相同的，包括广泛的细胞凋亡和口腔特异性 Wnt 信号通路的激活。截肢后 8 小时，Wnt 信号转导仅限于口腔再生。 Wnt 通路基因在刺伤中也上调，如果同时截除预先存在的组织者，这些伤口会诱导异位口腔结构的形成。我们的工作表明，口腔模式是作为水螅一般损伤反应的一部分而被激活的，而替代的损伤结果取决于来自周围组织的信号。此外，Wnt 信号传导可能是刺胞动物和两侧对称动物分裂之前保守的伤口反应的一部分。
During whole-body regeneration, a bisection injury can trigger two different types of regeneration. To understand the transcriptional regulation underlying this adaptive response, we characterized transcript abundance and chromatin accessibility during oral and aboral regeneration in the cnidarian Hydra vulgaris. We found that the initial response to amputation at both wound sites is identical and includes widespread apoptosis and the activation of the oral-specifying Wnt signaling pathway. By 8 hr post amputation, Wnt signaling became restricted to oral regeneration. Wnt pathway genes were also upregulated in puncture wounds, and these wounds induced the formation of ectopic oral structures if pre-existing organizers were simultaneously amputated. Our work suggests that oral patterning is activated as part of a generic injury response in Hydra, and that alternative injury outcomes are dependent on signals from the surrounding tissue. Furthermore, Wnt signaling is likely part of a conserved wound response predating the split of cnidarians and bilaterians.