Immune responses of ducks infected with duck Tembusu virus.
Immune responses of ducks infected with duck Tembusu virus.
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鸭天布苏病毒感染鸭的免疫反应
DOI：
10.3389/fmicb.2015.00425
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发表时间：
2015
影响因子：
5.2
通讯作者：
Wei L
中科院分区：
文献类型：
--
作者：
Li N;Wang Y;Li R;Liu J;Zhang J;Cai Y;Liu S;Chai T;Wei L
鸭坦布苏病毒（DTMUV）可引起鸭的严重疾病，其特征是产蛋量减少。虽然已经分离出病毒并开发了检测方法，但宿主对DTMUV感染的免疫反应尚不清楚。因此，我们系统地研究了DTMUV感染鸭的免疫相关基因的表达和病毒的分布，采用定量实时PCR。我们的研究结果表明，DTMUV在感染早期在许多组织中快速复制，感染后1天在脾脏中病毒滴度最高。Rig-1、Mda 5和Tlr 3参与DTMUV诱导的宿主免疫应答，并参与促炎细胞因子（IL-1β、IL-2、IL-6、Cxcl 8）和抗病毒蛋白（Mx、Oas等）的表达。在感染早期也被上调。IL-6的表达在所测试的组织中增加最显著。Mhc-I在脑和脾中表达上调，而Mhc-II在脑中表达上调，在脾中表达下调。脾组织中干扰素的表达也有不同程度的上调，而脑组织中的表达则不同。我们的研究表明DTMUV在各种组织中快速复制，并且宿主免疫反应在感染早期被激活。然而，细胞因子的过度表达可能会损害宿主。这些结果扩展了我们对鸭对DTMUV感染的免疫反应的理解，并提供了深入了解DTMUV的致病机制归因于宿主因素。
Duck Tembusu virus (DTMUV) can cause serious disease in ducks, characterized by reduced egg production. Although the virus has been isolated and detection methods developed, the host immune responses to DTMUV infection are unclear. Therefore, we systematically examined the expression of immune-related genes and the viral distribution in DTMUV-infected ducks, using quantitative real-time PCR. Our results show that DTMUV replicates quickly in many tissues early in infection, with the highest viral titers in the spleen 1 day after infection. Rig-1, Mda5, and Tlr3 are involved in the host immune response to DTMUV, and the expression of proinflammatory cytokines (Il-1β, –2, –6, Cxcl8) and antiviral proteins (Mx, Oas, etc.) are also upregulated early in infection. The expression of Il-6 increased most significantly in the tissues tested. The upregulation of Mhc-I was observed in the brain and spleen, but the expression of Mhc-II was upregulated in the brain and downregulated in the spleen. The expression of the interferons was also upregulated to different degrees in the spleen but that of the brain was various. Our study suggests that DTMUV replicates rapidly in various tissues and that the host immune responses are activated early in infection. However, the overexpression of cytokines may damage the host. These results extend our understanding of the immune responses of ducks to DTMUV infection, and provide insight into the pathogenesis of DTMUV attributable to host factors.
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