Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation
Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation
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DOI：
10.1016/s1097-2765(00)80179-3
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发表时间：
1999-01-01
期刊：
影响因子：
16
通讯作者：
Svejstrup, JQ
中科院分区：
文献类型：
--
作者：
Otero, G;Fellows, J;Svejstrup, JQ
分离了参与转录延伸的RNA聚合酶II(RNAPII)形式。延长的RNAPII与一种新的多亚单位复合体相关，称为延长子，其稳定的相互作用依赖于RNAPII羧基末端结构域(CTD)的过度磷酸化状态。还分离出了一种自由形式的延伸器，证明了复合体的离散性质，自由延伸器可以直接与RNAPII结合。克隆了延伸器最大亚基ELP1的编码基因。酵母elp1 Delta细胞的表型表明，在体内，伸长子参与了转录延伸和激活。我们的数据表明，从转录起始到延伸的转变涉及到在与CTD过度磷酸化耦合的反应中，多蛋白介体复合体交换为延伸子。
The form of RNA polymerase II (RNAPII) engaged in transcriptional elongation was isolated. Elongating RNAPII was associated with a novel multisubunit complex, termed elongator, whose stable interaction was dependent on a hyperphosphorylated state of the RNAPII carboxy-terminal domain (CTD). A free form of elongator was also isolated, demonstrating the discrete nature of the complex, and free elongator could bind directly to RNAPII. The gene encoding the largest subunit of elongator, ELP1, was cloned. Phenotypes of yeast elp1 Delta cells demonstrated an involvement of elongator in transcriptional elongation as well as activation in vivo. Our data indicate that the transition from transcriptional initiation to elongation involves an exchange of the multiprotein mediator complex for elongator in a reaction coupled to CTD hyperphosphorylation.