PHAGE ABORTIVE INFECTION MECHANISM FROM LACTOCOCCUS-LACTIS SUBSP LACTIS, EXPRESSION OF WHICH IS MEDIATED BY AN ISO-ISS1 ELEMENT
PHAGE ABORTIVE INFECTION MECHANISM FROM LACTOCOCCUS-LACTIS SUBSP LACTIS, EXPRESSION OF WHICH IS MEDIATED BY AN ISO-ISS1 ELEMENT
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DOI：
10.1128/aem.57.12.3547-3551.1991
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发表时间：
1991-12-01
影响因子：
4.4
通讯作者：
CHOPIN, MC
中科院分区：
文献类型：
--
作者：
CLUZEL, PJ;CHOPIN, A;CHOPIN, MC
从乳酸乳球菌亚种中克隆到一个5kb的噬菌体败育感染表型(Abi+)DNA片段。乳酸菌IL416。Abi+决定簇被亚克隆到一个2kb的片段上，该片段含有一个Iso-ISS1元件和一个753bp的开放阅读框，命名为ORFX。ORFX内的缺失导致Abi+表型的丢失，从而确定ORFX是结构性ABI-416基因。ABI-416的表达被证明是由Iso-ISS1元件介导的，该元件包含一个与革兰氏阳性启动子的共同序列相匹配的序列。
A 5-kb DNA fragment conferring a phage abortive infection phenotype (Abi+) has been cloned from Lactococcus lactis subsp. lactis IL416. The Abi+ determinant was subcloned on a 2-kb fragment which carried an Iso-ISS1 element and an open reading frame of 753 bp designated ORFX. Deletion within ORFX entailed the loss of the Abi+ phenotype, establishing that ORFX is the structural abi-416 gene. The expression of abi-416 was shown to be mediated by the Iso-ISS1 element, which contains a sequence fitting the consensus sequence for gram-positive promoters.