Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes
Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes
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DOI：
10.1038/385336a0
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发表时间：
1997-01-23
期刊：
影响因子：
64.8
通讯作者：
Nussenzweig, V
中科院分区：
文献类型：
--
作者：
Menard, R;Sultan, AA;Nussenzweig, V
疟疾寄生虫在蚊子载体中经历孢子生殖周期，子孢子，在血餐期间注入宿主的寄生虫的形式，在昆虫中肠的卵囊内发育，然后迁移到并最终侵入唾液腺，环子孢子蛋白（CS），唾液腺子孢子合成的主要蛋白之一，是一种表面相关分子，在子孢子对宿主的感染性中起重要作用（2）。在此，通过基因打靶，我们建立了伯氏疟原虫株系，其中单拷贝CS基因被破坏。CS（-）和野生型寄生虫在蚊子中肠中产生相似数量的大小相当的卵囊，然而，在CS（-）卵囊中，子孢子形成被严重抑制。因此，CS似乎具有多效性作用，并且对于媒介和宿主中的疟原虫都是至关重要的：在蚊子中，CS对于卵囊内的子孢子发育是必需的，并且在脊椎动物宿主中，它促进子孢子附着到肝细胞（3-7）。
Malaria parasites undergo a sporogonic cycle in the mosquito vector, Sporozoites, the form of the parasite injected into the host during a bloodmeal, develop inside oocysts in the insect midgut, then migrate to and eventually invade the salivary glands, The circumsporozoite protein (CS), one of the major proteins synthesized by salivary gland sporozoites', is a surface-associated molecule which is important in sporozoite infectivity to the host(2). Here, by gene targeting, we created Plasmodium berghei lines in which the single-copy CS gene was disrupted, The CS(-) and wild-type parasites produced similar numbers of oocysts of comparable size in the mosquito midgut, In the CS(-) oocysts, however, sporozoite formation was profoundly inhibited. CS therefore appears to have a pleiotropic role and to be vital for malaria parasites in both the vector and the host: in mosquitoes, CS is essential for sporozoite development within oocysts, and in the vertebrate host it promotes sporozoite attachment to hepatocytes(3-7).