Preparation of samples for single-worm tracking.
Preparation of samples for single-worm tracking.
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DOI：
10.1101/pdb.prot066993
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发表时间：
2011-12-01
影响因子：
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通讯作者：
Schafer, William R
中科院分区：
文献类型：
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作者：
Yemini, Eviatar;Kerr, Rex A;Schafer, William R
神经生物学研究在遗传易感生物很大程度上依赖于行为表型的稳健分析。秀丽隐杆线虫的简单身体结构使得它特别适合使用自动显微镜和图像分析来定量描述行为模式。本协议首先描述了用于生长和维持蠕虫跟踪的培养基的制备和使用。协议的第二部分描述了如何在视频分析期间准备单个年轻成虫进行记录。虽然该协议是为单蠕虫跟踪器开发的，但它解决了在所有系统中生成可复制的标准化图像的重要因素。
Neurobiological research in genetically tractable organisms relies heavily on robust assays for behavioral phenotypes. The simple body plan of the nematode Caenorhabditis elegans makes it particularly amenable to the use of automated microscopy and image analysis to describe behavioral patterns quantitatively. This protocol first describes the preparation and use of media for growing and maintaining worms for tracking. The second part of the protocol describes how to prepare a single young adult worm for recording during video analysis. Although the protocol was developed for use in a single-worm tracker, it addresses factors important for the generation of reproducible, standardized images in all systems.