SELECTIVE RELEASE OF SPERMINE AND SPERMIDINE FROM THE RAT STRIATUM BY N-METHYL-D-ASPARTATE RECEPTOR ACTIVATION INVIVO
SELECTIVE RELEASE OF SPERMINE AND SPERMIDINE FROM THE RAT STRIATUM BY N-METHYL-D-ASPARTATE RECEPTOR ACTIVATION INVIVO
复制标题
DOI：
10.1111/j.1471-4159.1992.tb10960.x
复制
发表时间：
1992-06-01
影响因子：
4.7
通讯作者：
CARTER, C
中科院分区：
文献类型：
--
作者：
FAGE, D;VOLTZ, C;CARTER, C
通过透析插管向麻醉大鼠纹状体内输注 N-甲基-D-天冬氨酸（NMDA；250-1,000-mu-M），导致透析液中回收的精胺和亚精胺浓度显着且快速增加。 NMDA 释放的精胺和亚精胺的细胞外浓度经计算处于低微摩尔范围内。腐胺水平未受 NMDA 显着影响。 NMDA (500-mu-M) 的作用被先前全身注射 MK-801 (3 mg/kg, i.p.) 阻断，但对纹状体内输注河豚毒素 (1-mu-M) 不敏感。向纹状体内注入红藻氨酸或使君子酸盐（1,000-mu-M）不会增加透析液中的多胺水平。通过输注高浓度 K+ (> 100 mM)，精胺和亚精胺透析液水平也显着增加，尽管 K+ 的影响远不如 NMDA 显着。纹状体多胺通过 NMDA 受体激活特异性释放到细胞外空间。由于其对受体和电压控制的阳离子通道具有多种作用，NMDA 受体激活释放的多胺可能在已经归因于 NMDA 受体刺激的现象中发挥重要作用，例如长时程增强、突触可塑性和神经毒性。
The intrastriatal infusion of N-methyl-D-aspartate (NMDA; 250-1,000-mu-M) via a dialysis cannula in anesthetized rats resulted in a marked and rapid increase in the concentrations of spermine and spermidine recovered in the dialysate. Extracellular concentrations of NMDA-released spermine and spermidine were calculated to be in the low micromolar range. Putrescine levels were not significantly affected by NMDA. The effects of NMDA (500-mu-M) were blocked by the previous systemic injection of MK-801 (3 mg/kg, i.p.) but were insensitive to the intrastriatal infusion of tetrodotoxin (1-mu-M). Intrastriatally infused kainate or quisqualate (1,000-mu-M) did not increase polyamine levels in the dialysate. Spermine and spermidine dialysate levels were also significantly increased by the infusion of high concentrations of K+ (> 100 mM), although the effects of K+ were considerably less marked than those of NMDA. Striatal polyamines are released into the extracellular space specifically by NMDA receptor activation. Because of their multiple effects on receptor- and voltage-operated cation channels, polyamines that are released by NMDA receptor activation may play an important role in phenomena already attributed to NMDA receptor stimulation, such as long-term potentiation, synaptic plasticity, and neurotoxicity.