Selenomethionine exposure affects chondrogenic differentiation and bone formation in Japanese medaka (Oryzias latipes).
Selenomethionine exposure affects chondrogenic differentiation and bone formation in Japanese medaka (Oryzias latipes).
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DOI：
10.1016/j.jhazmat.2019.121720
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发表时间：
2019-11
影响因子：
13.6
通讯作者：
Huan Wang;Hongxing Chen;Melissa Chernick;Dan Li;G. Ying;Jingfeng Yang;Na Zheng;Lingtian Xie
中科院分区：
文献类型：
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作者：
Huan Wang;Hongxing Chen;Melissa Chernick;Dan Li;G. Ying;Jingfeng Yang;Na Zheng;Lingtian Xie
由于人类活动而进入水环境的过量硒与鱼类的发育异常有关，包括头部和脊柱的骨骼畸形。然而，这种发育毒性的机制尚未得到充分表征。在这项研究中，日本青鳉（Oryzias latipes）胚胎暴露于硒-L-蛋氨酸（Se-Met）在一系列浓度范围内。性别决定区Y（SRY）相关框（Sox 9a和Sox 9b），runt相关转录因子2（Runx 2）和褪黑激素受体（Mtr）的基因表达进行了评估。在受精后10天和22天（dpf）的骨骼染色中观察到美克尔软骨长度、尾曲和钙化减少的变化。胚胎暴露于Osterix-mCherry转基因青鳉导致牙齿数量减少，Sox9a和Sox9b在孵化前被Se-Met上调，Runx2和Mtr在孵化前被Se-Met下调。全组织原位杂交（WISH）将基因表达定位于体内观察到的受影响区域。此外，硒-蛋氨酸暴露的α Mtrmorpholino（Mtr-MO）以及Luzindole暴露的胚胎发育相似的骨骼畸形，支持参与的Mtr。这些研究结果表明，硒蛋氨酸调节参与软骨分化和骨形成在发育过程中的关键基因的表达。
Excess selenium entering the aquatic environment from anthropogenic activities has been associated with developmental abnormalities in fish including skeletal deformities of the head and spine. However, mechanisms of this developmental toxicity have not been well-characterized. In this study, Japanese medaka (Oryzias latipes) embryos were exposed to seleno-l-methionine (Se-Met) in a range of concentrations. Gene expression was evaluated for sex-determining region Y (SRY)-related box (Sox9aandSox9b), runt-related transcription factor 2 (Runx2), and melatonin receptor (Mtr). Alterations in the length of Meckel’s cartilage, tail curvature, and decreased calcification were observed in skeletal stains at 10- and 22-days post-fertilization (dpf). Embryonic exposure ofOsterix-mCherry transgenic medaka resulted in fewer teeth.Sox9aandSox9bwere up-regulated, whileRunx2andMtrwere down-regulated by Se-Met prior to hatch. Whole mountin situhybridization (WISH) localized gene expression to areas observed to be affectedin vivo. In addition, Se-Met exposures of aMtrmorpholino (Mtr-MO) as well as Luzindole exposed embryos developed similar skeletal malformations, supporting involvement ofMtr. These findings demonstrate that Se-Met modulates expression of key genes involved in chondrogenic differentiation and bone formation during development.