[Increased serum levels of human cardiac myosin light chain 1 in patients with renal failure].
[Increased serum levels of human cardiac myosin light chain 1 in patients with renal failure].
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肾衰竭患者血清人心肌肌球蛋白轻链1水平升高[J].
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
N. Sugino
中科院分区：
文献类型：
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作者：
K. Nakai;K. Nakai;C. Itoh;M. Kikuchi;S. Nakamura;J. Kamata;K. Hiramori;W. Yumura;S. Horita;N. Sugino
本研究采用单克隆抗体酶免疫分析法（EIA）测定了急性心肌梗死（AMI）、慢性肾功能衰竭（CRF）和不同程度肾功能不全（RD）患者血清和尿液中cMLC 1的浓度，并与正常对照组进行比较，试图阐明肾功能衰竭患者血清cMLC 1水平升高的机制。CRF维持性血液透析（HD）患者血清cMLC 1水平为20.3 ± 19.6ng/ml，明显高于正常对照组（0.54 ± 0.55ng/ml）。保守治疗的RD和CRF患者血清cMLC 1水平明显高于正常对照组，尤以晚期CRF患者为甚，但各指标与Ccr无相关性。仅2例AMI合并CRF或RD患者尿中可检出cMLC 1。此外，RD患者肾活检标本的免疫组织学研究未显示cMLC 1在肾小球中沉积。这些结果表明，cMLC 1可通过肾小球过滤，然后在肾小管中吸收。
In this study, we measured cMLC1 concentration in serum and urine from patients with acute myocardial infarction (AMI), chronic renal failure (CRF), and various grades of renal dysfunction (RD) in comparison with normal controls, by using enzyme immunoassay (EIA) with monoclonal antibody, and attempted to elucidate the mechanism of increased serum level of cMLC1 in patients with renal failure. The serum level of cMLC1 of CRF patients under maintenance hemodialysis (HD) was 20.3 +/- 19.6 ng/ml, markedly higher than normal controls (0.54 +/- 0.55 ng/ml). The patients with RD and CRF under conservative therapy had higher serum cMLC1 level than normal controls especially in advanced CRF, while each value not correlating with their creatine clearance (Ccr). cMLC1 in urine was detectable in only two cases with AMI accompanied with CRF or RD. In addition, immunohistological studies of renal biopsy specimens from RD patients did not show cMLC1 deposits in glomerulus. These results suggest that cMLC1 is assumably filtered through the glomerulus, and then absorbed in the renal tubule.