Novel gelatin-PHEMA porous scaffolds for tissue engineering applications
Novel gelatin-PHEMA porous scaffolds for tissue engineering applications
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DOI：
10.1039/c2sm25536g
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Stancu, Izabela-Cristina
中科院分区：
文献类型：
--
作者：
Dragusin, Diana-Maria;Van Vlierberghe, Sandra;Stancu, Izabela-Cristina
在目前的工作中，新型的双组分聚合物水凝胶的基础上，甲基丙烯酰胺改性明胶（MAG）和2-羟乙基甲基丙烯酸酯（HEMA）已通过交联聚合使用光引发。使用1/0.5和1/10 w/w之间的不同MAG/HEMA比率制备了五种类型的新型水凝胶。随后，通过低温处理，然后冷冻干燥获得多孔支架。进行了物理化学测量以及体外降解试验，以将材料组成与相应性能相关联。在所研究的特性中，我们必须提到吸水能力、流变特性和酶介导的降解行为。结果表明，初始聚合混合物中的HEMA含量调节多孔支架的结构，从具有最低HEMA含量的水凝胶的直接的、从上到下定向的通道到通道的复杂且致密的内部孔隙率（在HEMA负载量较高的材料的情况下）。在针对组织工程应用的同时，重要的是要注意共价结合的明胶序列显著改善PHEMA基水凝胶的生物相容性。
In the present work, novel bicomponent polymeric hydrogels based on methacrylamide-modified gelatin (MAG) and 2-hydroxyethyl methacrylate (HEMA) have been prepared by cross-linking polymerization using photoinitiation. Five types of novel hydrogels have been prepared using different MAG/HEMA ratios between 1/0.5 and 1/10 w/w. Subsequently, porous scaffolds were obtained via a cryogenic treatment followed by freeze-drying. Physico-chemical measurements as well as in vitro degradation tests have been performed in order to correlate the material composition with the corresponding properties. Among the properties studied we have to mention the water uptake capacity, the rheological properties and the enzyme-mediated degradation behaviour. The results indicate that the HEMA content in the initial polymerization mixtures modulates the architecture of the porous scaffolds from straightforward, top-to-bottom oriented channels for hydrogels possessing the lowest HEMA content to a complex and dense internal porosity of the channels the case of higher HEMA loaded materials. While aiming at tissue engineering applications, it is important to notice that the covalently bound gelatin sequences significantly improve the biocompatibility of PHEMA based hydrogels.