Involvement of gibberellin and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota).
Involvement of gibberellin and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota).
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DOI：
10.1078/0176-1617-00892
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发表时间：
2003
影响因子：
4.3
通讯作者：
Y. Tokuji;K. Kuriyama
中科院分区：
文献类型：
--
作者：
Y. Tokuji;K. Kuriyama
胡萝卜的体细胞胚胎发生是植物细胞全能性的一个典型例子。然而，对体细胞向胚胎细胞转变的过程知之甚少。为了验证植物激素在胚胎发生能力获得过程中的作用，我们研究了植物生长调节剂及其抑制剂对生长素诱导的胡萝卜直接体细胞胚胎发生的影响。赤霉素（Gibberellin， GA）抑制早期胚性细胞向球形分化发育，抑制赤霉素合成的单唑唑（uniconazole）促进初生胚胎向次生胚胎发育。嘌呤核苷是一种抗细胞分裂素，可以抑制体细胞胚胎的直接发生，而这种作用被细胞分裂素（CK）的应用所抵消。上述结果表明，GA和CK对生长素诱导的胡萝卜体细胞胚胎发生的早期发育具有调控作用。
Somatic embryogenesis of carrots is a typical example of the totipotency of plant cells. However, little is known about the process of change from somatic cells to embryogenic cells. To test the involvement of plant hormones in the acquisition process of embryogenic potency, we investigated the effects of plant growth regulators and their inhibitors on auxin-induced direct somatic embryogenesis of carrots. Gibberellin (GA) inhibited the early stage of embryogenic cell differentiation/development to the globular stage and uniconazole, an inhibitor of GA synthesis, promoted the secondary embryogenesis from the primary embryo. Purine riboside, an anticytokinin, inhibited direct somatic embryogenesis, and this effect was nullified by the application of cytokinin (CK). These results show that GA and CK regulate the early stage of auxin-induced somatic embryogenesis in carrots.