A proteomics approach to quantify protein levels following RNA interference:: Case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans
A proteomics approach to quantify protein levels following RNA interference:: Case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans
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DOI：
10.1021/pr8001035
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Brophy, P. M.
中科院分区：
文献类型：
--
作者：
LaCourse, E. J.;Perally, S.;Brophy, P. M.
通过干扰RNA(RNAi)技术进行功能缺失表型分析是确定基因功能的一种革命性方法。虽然基于转录本的方法通常验证RNAi基因抑制研究，但基于蛋白质的验证较少开发。这份报告说明了十二烷基硫酸钠双向凝胶电泳(2-DE)和凝胶分析在RNAi后定量蛋白质水平的可能性。这一案例研究涉及模式生物秀丽线虫的三个谷胱甘肽转移酶(GST)基因。
Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.