Electrochemical determination of atropine at multi-wall carbon nanotube electrode based on the enhancement effect of sodium dodecyl benzene sulfonate
Electrochemical determination of atropine at multi-wall carbon nanotube electrode based on the enhancement effect of sodium dodecyl benzene sulfonate
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DOI：
10.1016/j.colsurfb.2011.10.020
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发表时间：
2012-03-01
影响因子：
5.8
通讯作者：
Pitre, Krishna Sadashiv
中科院分区：
文献类型：
--
作者：
Dar, Riyaz Ahmad;Brahman, Pradeep Kumar;Pitre, Krishna Sadashiv
基于阴离子表面活性剂十二烷基苯磺酸钠(SDBS)对阿托品的增敏作用，建立了测定阿托品的电化学方法。在pH 10.5的四甲基氢氧化铵支持电解液中，在0.4×10(-4)M的SOBS存在下，阿托品在多壁碳纳米管电极(MWCNTE)上产生一个清晰灵敏的氧化峰。与无SOBS时相比，有SOBS时阿托品的氧化峰电流显著增大。对阿托品测定的支持电解质、悬浮液浓度、蓄积时间等实验参数进行了优化。氧化峰电流与阿托品的浓度在3.98~27.23 ng/ml范围内呈良好的线性关系，检测下限为0.449 ng/ml。将该伏安法用于印度中药材曼陀罗种子和叶中阿托品的测定，获得了满意的回收率。所建立的方法也可用于眼药液(滴眼液)制剂中阿托品的分析。阿托品的日内和日间分析的相对标准偏差分别为0.67%和0.86%(n=3)，累积时间为120 S。(C)2011年爱思唯尔公司保留所有权利。
Herein, a new electrochemical method was described for the determination of atropine based on the enhancement effect of an anionic surfactant: sodium dodecyl benzene sulfonate (SDBS). In pH 10.5 tetramethyl ammonium hydroxide as supporting electrolyte and in the presence of 0.4 x 10(-4) M SOBS, atropine yields a well-defined and sensitive oxidation peak at the multi-wall carbon nanotube electrode (MWCNTE). Compared with that in the absence of SOBS, the oxidation peak current of atropine remarkably increases in the presence of SOBS. The experimental parameters, such as supporting electrolyte, concentration of SOBS, and accumulation time, were optimized for atropine determination. The oxidation peak current is proportional to the concentration of atropine over the range from 3.98 ng/ml to 27.23 ng/ml. The detection limit is 0.449 ng/ml after 2 min of accumulation. This new voltammetric method was successfully used to determine atropine in Indian traditional medicine (seeds and leaves of Datura stramonium) with satisfactory recoveries. The developed method was also used for the analysis of atropine in pharmaceutical formulation of ophthalmic solution (eye drop). The relative standard deviations of intraday and interday analyses for atropine were 0.67% and 0.86% respectively (n = 3) for the accumulation time of 120 s. (C) 2011 Elsevier B.V. All rights reserved.