High prevalence of methicillin resistant Staphylococcus aureus in pigs
High prevalence of methicillin resistant Staphylococcus aureus in pigs
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DOI：
10.1016/j.vetmic.2007.01.027
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发表时间：
2007-06-21
影响因子：
3.3
通讯作者：
Huijsdens, X. W.
中科院分区：
文献类型：
--
作者：
de Neeling, A. J.;van den Broek, M. J. M.;Huijsdens, X. W.
最近在荷兰从猪和养猪户中分离出耐甲氧西林金黄色葡萄球菌（MRSA）。为了评估MRSA在荷兰猪群中的传播情况，我们在9个屠宰场筛选了540头猪，2005年屠宰了具有代表性的荷兰猪（63%）。我们发现209头（39%）猪的名字中携带MRSA。54组连续10头猪中有44组（81%）受到影响，每组来自不同的农场，所有屠宰场。所有MRSA分离株均属于1个克隆群，多位点序列型398和密切相关的spa型（主要为t011、t108和t1254）。葡萄球菌染色体盒（SCCmec）有三种类型：III型（3%）、IVa型（39%）和V型（57%）。所有44株检测菌株（每组1株）均对四环素耐药，反映了四环素在养猪业中的高用量和优势用量。23%的分离株对红霉素和克林霉素都耐药，36%的分离株对卡那霉素、庆大霉素和妥布霉素耐药，但只有一株分离株对复方新诺明耐药，对环丙沙星和其他几种抗生素没有耐药。MRSA阳性猪的百分比在屠宰场之间和屠宰场内的群体之间存在显著差异，表明从农场交付的猪中MRSA的高流行率以及屠宰场的交叉污染。(c) 2007 Elsevier B.V.版权所有
Recently methicillin resistant Staphylococcus aureus (MRSA) was isolated from pigs and pig farmers in The Netherlands. In order to assess the dissemination of MRSA in the Dutch pig population, we screened 540 pigs in 9 slaughterhouses, where a representative portion of Dutch pigs (63%) was slaughtered in 2005. We found 209 (39%) of the pigs to carry MRSA in their nares. Forty-four of 54 groups of 10 consecutive pigs (81 %), each group from a different farm, and all slaughterhouses were affected.All MRSA isolates belonged to 1 clonal group, showing Multi-Locus Sequence Type 398 and closely related spa types (mainly t011, t108 and t1254). Three types of the Staphylococcal Chromosome Cassette (SCCmec) were found: III (3%), IVa (39%) and V (57%). All 44 tested isolates (1 isolate per group) were resistant to tetracycline, reflecting the high and predominant use of tetracyclines in pig husbandry. Twenty-three percent of the isolates were resistant to both erythromycin and clindamycin and 36% to kanamycin, gentamicin and tobramycin but only a single isolate was resistant to co-trimoxazole and none to ciprofloxacin and several other antibiotics.The percentage of MRSA positive pigs was significantly different among slaughterhouses and among groups within slaughterhouses, indicating a high prevalence of MRSA in pigs delivered from the farms as well as cross contamination in the slaughterhouses. (c) 2007 Elsevier B.V. All rights reserved.