Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model.
Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model.
复制标题
DOI：
10.3389/fonc.2023.1114406
复制
发表时间：
2023
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
评估兔 VX2 肝肿瘤中微血管密度 (MVD) 和体素内不相干运动 (IVIM) 磁共振成像 (MRI) 参数之间的相关性以及经动脉化疗栓塞 (TACE) 后糖酵解通量的影响。 15只新西兰白兔的VX2肝肿瘤同种异体移植物用无菌生理盐水（对照组，n = 5）或碘油-阿霉素乳剂（实验组，n = 10）治疗。术后 2 周进行 MRI 评估 IVIM 参数，包括表观扩散系数 (ADC)、纯扩散系数 (D)、伪扩散系数 (D*) 和灌注分数 (PF)。所有动物样本均取自肿瘤和周围肝脏。 CD31、CD34、CD105 和 VEGF 的免疫染色用于评估 MVD。使用蛋白质印迹法测定 Glut4、HK2、PKM2、LDHA 和 MCT1 的蛋白表达。采用Pearson相关性检验分析MVD与IVIM参数之间的关系。瘤周区域的 D* 值与 CD34 呈负相关 (r = –0.71, P = 0.01)。瘤周区域PF值与CD34（r = 0.68，P = 0.015）、CD105（r = 0.76，P = 0.004）和VEGF（r = 0.72，P = 0.008）呈正相关。实验组瘤周区Glut4、HK2、PKM2、MCT1高于对照组（均P < 0.05）。在兔肝肿瘤模型中，IVIM 参数与 TACE 后瘤内和瘤周区域的 MVD 相关。 MVD反映的血管生成可能与糖酵解通量的变化有关。
To evaluate the correlation between microvascular density (MVD) and intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) parameters and the effect of glycolytic flux after transarterial chemoembolization (TACE) in a rabbit VX2 liver tumor. VX2 liver tumor allografts in 15 New Zealand white rabbits were treated with sterile saline (control group, n = 5) or lipiodol-doxorubicin emulsion (experimental group, n = 10). MRI was performed 2 weeks after the procedure to evaluate IVIM parameters, including apparent diffusion coefficient (ADC), pure diffusion coefficient (D), pseudodiffusion coefficient (D*), and perfusion fraction (PF). All animal samples were taken of the tumor and surrounding liver. Immunostaining for CD31, CD34, CD105, and VEGF was used to evaluate MVD. The protein expression of Glut4, HK2, PKM2, LDHA, and MCT1 was determined using western blotting. Pearson correlation tests were used to analyze the relationship between MVD and IVIM parameters. D* value in the peritumoral region was negatively correlated with CD34 (r = –0.71, P = 0.01). PF value positively correlated with CD34 (r = 0.68, P = 0.015), CD105 (r = 0.76, P = 0.004) and VEGF (r = 0.72, P = 0.008) in the peritumoral region. Glut4, HK2, PKM2, and MCT1 in the peritumoral regions were higher in the experimental group than in the control group (all P < 0.05). IVIM parameters were correlated with MVD in the intratumoral and peritumoral regions after TACE in a rabbit liver tumor model. The angiogenesis reflected by MVD may be related to changes of glycolytic flux.
登录
查看更多内容
影响因子：
4.7
作者：
Han, Yonglai;Ma, Jinning;Li, Yuqing
通讯作者：
Li, Yuqing
影响因子：
4
作者：
Lin, Min;Tian, Man-Man;Jin, Ping
通讯作者：
Jin, Ping
影响因子：
4.7
作者：
Liang, Jianye;Li, Zhipeng;Xie, Chuanmiao
通讯作者：
Xie, Chuanmiao
影响因子：
4.3
作者：
Ranieri, Girolamo;Ammendola, Michele;Gadaleta, Cosmo Damiano
通讯作者：
Gadaleta, Cosmo Damiano
影响因子：
2.9
作者：
通讯作者：
--